Pancreatic alpha-amylase Monoklonaler Antikörper

Pancreatic alpha-amylase Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

2F1G8

Kat-Nr. : 66133-1-Ig

Synonyme

AMY2A, Amylase Alpha, Amylase, alpha, AMY2B, AMY2

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  WB analysis of human saliva using 66133-1-Ig

  human saliva were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human saliva using 66133-1-Ig

  0.1 μL human saliva lysate were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig pancreas using 66133-1-Ig

  0.6 μg pig pancreas tissue lysate were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 66133-1-Ig

  0.6 μg rat pancreas tissue lysate were subjected to SDS PAGE followed by western blot with 66133-1-Ig (Amylase, alpha antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 66133-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66133-1-Ig (Amylase, alpha antibody at dilution of 1:50(under 10x lens).

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  IHC staining of human pancreas cancer using 66133-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66133-1-Ig (Amylase, alpha antibody at dilution of 1:50(under 40x lens).

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  IF Staining of BxPC-3 using 66133-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using Amylase Alpha antibody (66133-1-Ig, Clone: 2F1G8 ) at dilution of 1:1200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human saliva, Hausschwein-Pankreasgewebe, Ratten-Pankreasgewebe, Gewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: BxPC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:600-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

66133-1-Ig bindet in WB, IHC, IF/ICC, ELISA Pancreatic alpha-amylase und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Pancreatic alpha-amylase fusion protein Ag8819
• Vollständiger Name: amylase, alpha 2A (pancreatic)
• Berechnetes Molekulargewicht: 511 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC007060
• Gene symbol: Amylase Alpha
• Gene ID (NCBI): 279
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The complete human amylase gene contains five intact genes: two pancreatic genes (AMY2A and AMY2B) and three salivary genes (AMYIA, AMYIB, and AMYIC). AMY2A(Pancreatic alpha-amylase)is also named as PA and is a 56 kDa protein consisting of 496 amino acids in a single polypeptide chain, folded into three domains(PMID:10657258). It has a hydrolase activity acting on glycosyl bonds. AMY2A and AMY2B transcripts are present in the human pancreas but not in the parotid salivary gland, while AMY1 transcripts are present in the parotid gland but not in the pancreas(PMID:1692956).This antibody can recognize amylase alpha.

Publikationen

Species	Application	Title
	IF	Am J Physiol Gastrointest Liver Physiol; Is the intestinal mucous layer a NAtural Deep Eutectic Solvent (NADES) based digestion matrix; Authors - Theo A T G van Kempen
human	IHC	J Toxicol Pathol; Pancreatic Ductal Adenocarcinoma in a Wistar Hannover GALAS Rat.; Authors - Yusuke Kuroda
pig	IHC	Biochem Biophys Rep; Multiple cystic sphere formation from PK-8 cells in three-dimensional culture; Authors - Yuuki Shichi
human	WB	J Proteome Res; Proteomic Analysis of Differences in the Freezability of Porcine Sperm Identifies α-Amylase As a Key Protein; Authors - Kejun Wang