AMZ2 Polyklonaler Antikörper

AMZ2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16664-1-AP

Synonyme

Archaemetzincin 2, Archaemetzincin-2, Archeobacterial metalloproteinase-like protein 2, BM-014, EC:3.4.-.-

---

• 

  WB analysis of HepG2 using 16664-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16664-1-AP (AMZ2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16664-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16664-1-AP (AMZ2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16664-1-AP (AMZ2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 16664-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16664-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16664-1-AP (AMZ2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 16664-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug AMZ2 Polyclonal antibody (16664-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Hirngewebe, humanes Herzgewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Hautgewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16664-1-AP bindet in WB, IHC, IF/ICC, ELISA AMZ2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AMZ2 fusion protein Ag10141
• Vollständiger Name: archaelysin family metallopeptidase 2
• Berechnetes Molekulargewicht: 360 aa, 41 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC056271
• Gene symbol: AMZ2
• Gene ID (NCBI): 51321
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AMZ2(archaelysin family metallopeptidase 2) is a zinc metalloprotease. Its calculated molecular weight and observed molecular weight are both 41kDa.