ANP32A Polyklonaler Antikörper

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• KD/KO Validated

ANP32A Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 15810-1-AP

Synonyme

MAPM, Leucine-rich acidic nuclear protein, LANP, I1PP2A, C15orf1

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  WB analysis using 15810-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15810-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 15810-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15810-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15810-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15810-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15810-1-AP (ANP32A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 15810-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15810-1-AP (ANP32A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 15810-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15810-1-AP (ANP32A Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 15810-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 15810-1-AP (ANP32A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A2780 using 15810-1-AP

  Immunofluorescent analysis of A2780 cells, using ANP32A antibody 15810-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293T-Zellen, HeLa-Zellen, humanes Hirngewebe, MCF-7-Zellen, Maushirngewebe, Maus-Thymusgewebe, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen, A2780-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below

Produktinformation

15810-1-AP bindet in WB, IHC, IF/ICC, ELISA ANP32A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANP32A fusion protein Ag8546
• Vollständiger Name: acidic (leucine-rich) nuclear phosphoprotein 32 family, member A
• Berechnetes Molekulargewicht: 249 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC007200
• Gene symbol: ANP32A
• Gene ID (NCBI): 8125
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANP32A, an acidic leucine-rich nuclear phosphoprotein, is a member of the inhibitor of the histone acetyltransferase (INHAT) complex and preferentially binds to unmodified histone H3 tails in vitro. ANP32A is expressed in normal tissues as well as in pancreatic, breast and prostate cancer and other malignant tumors. ANP32A plays an important role in cell proliferation, apoptosis, transcriptional regulation, signal transduction and other processes.

Publikationen

Species	Application	Title
human,chicken	WB	Sci Adv; Avian ANP32A incorporated in avian influenza A virions promotes interspecies transmission by priming early viral replication in mammals; Authors - Lei Na
human	WB	Cancer Cell Int; Super enhancer related gene ANP32B promotes the proliferation of acute myeloid leukemia by enhancing MYC through histone acetylation; Authors - Xiaomei Wan ; KD Validated
human	WB	Arch Virol; The interaction of cellular protein ANP32A with influenza A virus polymerase component PB2 promotes vRNA synthesis.; Authors - Xiuli Wei ; KD Validated
human	WB	Nat Commun; Human ANP32A/B are SUMOylated and utilized by avian influenza virus NS2 protein to overcome species-specific restriction; Authors - Liuke Sun
mouse	WB	Biomedicines; The Effect of Tff3 Deficiency on the Liver of Mice Exposed to a High-Fat Diet; Authors - Iva Bazina