ANTXR1 Polyklonaler Antikörper

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• KD/KO Validated

ANTXR1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 15091-1-AP

Synonyme

TEM8, Tumor endothelial marker 8, ATR, Anthrax toxin receptor 1

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  WB analysis of mouse testis using 15091-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15091-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-13 using 15091-1-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15091-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15091-1-AP (TEM8 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 15091-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15091-1-AP (TEM8 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 15091-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15091-1-AP (TEM8 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 15091-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15091-1-AP (TEM8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, HeLa-Zellen, Mauslungengewebe, PC-13-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

15091-1-AP bindet in WB, IHC, IF/ICC, ELISA ANTXR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANTXR1 fusion protein Ag4626
• Vollständiger Name: anthrax toxin receptor 1
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 85 kDa, 80 kDa
• GenBank-Zugangsnummer: BC012074
• Gene symbol: ANTXR1
• Gene ID (NCBI): 84168
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tumor endothelial marker 8 (TEM8, ANTXR1) and capillary morphogenesis protein 2 (CMG2, ANTXR2) are the two well-characterized anthrax toxin receptors, each containing a von Willebrand factor A (vWA) domain responsible for anthrax protective antigen (PA) binding (PMID: 23271637). TEM8 is a highly-conserved single-pass cell-surface protein overexpressed on tumor-infiltrating vasculature, and is thought to play a positive role in endothelial cell activities related to angiogenesis (PMID: 11559528; 15777794 ). The long isoforms of TEM8 have been identified as an 80-85 kDa doublet, and experimental deglycosylation reduced the molecular mass to 70 kDa, indicating that TEM8 undergoes multiple glycosylations (PMID: 14871805; 15689409).

Publikationen

Species	Application	Title
human	WB,IHC	Am J Transl Res; MicroRNA-26b-3p/ANTXR1 signaling modulates proliferation, migration, and apoptosis of glioma.; Authors - Fei Geng
human	WB	Cancer Manag Res; KLF7 Promotes Gastric Carcinogenesis Through Regulation of ANTXR1.; Authors - Yuanchun Li ; KD Validated
human	WB	Comput Math Methods Med; miR-381-3p Involves in Glioma Progression by Suppressing Tumor-Promoter Factor ANTXR1.; Authors - Zhiqiang Dong
mouse	WB,IF	J Cell Physiol; Mechanical strain regulates osteogenesis via Antxr1/LncRNA H19/Wnt/β-catenin axis; Authors - Lifei Liu