Annexin A1 Polyklonaler Antikörper

Annexin A1 Polyklonal Antikörper für FC, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 55018-1-AP

Synonyme

Annexin 1, annexin A1, Annexin I, ANX1, ANXA1, Calpactin 2, Calpactin II, Chromobindin 9, Lipocortin I, LPC1, p35

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Galerie der Validierungsdaten

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  WB analysis of C2C12 using 55018-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 55018-1-AP (Annexin A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55018-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55018-1-AP (Annexin A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55018-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55018-1-AP (Annexin A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 55018-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 55018-1-AP (Annexin A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 55018-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 55018-1-AP (Annexin A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 55018-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55018-1-AP (Annexin A1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus using 55018-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 55018-1-AP (Annexin A1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human oesophagus using 55018-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 55018-1-AP (Annexin A1 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 55018-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55018-1-AP (Annexin A1 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 55018-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55018-1-AP (Annexin A1 antibody at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HeLa using 55018-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Annexin A1 antibody (55018-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C2C12 cell, C6-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Ösophagusgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

55018-1-AP bindet in WB, IHC, IF, FC, ELISA Annexin A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: annexin A1
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: NM_000700
• Gene symbol: ANXA1
• Gene ID (NCBI): 301
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes. The antibody is specific to ANXA1.

Publikationen

Species	Application	Title
		Expert Rev Mol Diagn; Co-overexpression of Hsp90-β and annexin A1 with a significantly positive correlation contributes to the diagnosis of lung cancer.; Authors - Rong Biaoxue
mouse	IF	J Cell Sci; Platelet-derived microvesicles regulate vascular smooth muscle cell energy metabolism via PRKAA after intimal injury.; Authors - Jing Yan
monkey	WB	J Proteomics; iTRAQ-based comparative proteomic analysis of Vero cells infected with virulent and CV777 vaccine strain-like strains of porcine epidemic diarrhea virus.; Authors - Xiaozhen Guo
mouse	WB,IF	Mediators Inflamm; Interaction between ANXA1 and GATA-3 in Immunosuppression of CD4(+) T Cells.; Authors - Peng Huang
human	WB, IHC	J Exp Clin Cancer Res; Upregulation of Hsp90-beta and annexin A1 correlates with poor survival and lymphatic metastasis in lung cancer patients.; Authors - Biaoxue Rong R
human	IHC	Am J Transl Res; Elevated serum annexin A1 as potential diagnostic marker for lung cancer: a retrospective case-control study.; Authors - Biaoxue Rong