Annexin A1 Monoklonaler Antikörper

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• KD/KO Validated

Annexin A1 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(10)

CloneNo.

1E1B7

Kat-Nr. : 66344-1-Ig

Synonyme

ANXA1, 1E1B7, Annexin 1, Annexin Ac2-26, Annexin I

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  WB analysis using 66344-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66344-1-Ig (Annexin A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66344-1-Ig

  WB result of Annexin A1 antibody (66344-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin A1 transfected HeLa cells.

• 

  WB analysis using 66344-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66344-1-Ig (Annexin A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66344-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66344-1-Ig (Annexin A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66344-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66344-1-Ig (Annexin A1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 66344-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 66344-1-Ig (Annexin A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 66344-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66344-1-Ig (Annexin A1 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 66344-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66344-1-Ig (Annexin A1 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 66344-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66344-1-Ig (Annexin A1 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 66344-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66344-1-Ig (Annexin A1 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66344-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66344-1-Ig (Annexin A1 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66344-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66344-1-Ig (Annexin A1 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 66344-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66344-1-Ig (Annexin A1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 66344-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66344-1-Ig (Annexin A1 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A549 using 66344-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Annexin A1 antibody (66344-1-Ig, Clone: 1E1B7 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

• 

  FC experiment of K-562 using 66344-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.8 ug Anti-Human Annexin A1 (66344-1-Ig, Clone:1E1B7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, 4T1-Zellen, A431-Zellen, HCT 116-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe, humanes Lebergewebe, humanes Pankreasgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen
• Erfolgreiche Detektion in FC (Intra): K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Produktinformation

66344-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Annexin A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Annexin A1 fusion protein Ag17273
• Vollständiger Name: annexin A1
• Berechnetes Molekulargewicht: 346 aa, 39 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC001275
• Gene symbol: Annexin A1
• Gene ID (NCBI): 301
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.

Publikationen

Species	Application	Title
mouse	IF	Adv Sci (Weinh); M2 Macrophage-Derived Extracellular Vesicles Reprogram Immature Neutrophils into Anxa1hi Neutrophils to Enhance Inflamed Bone Regeneration; Authors - Yufei Yao
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
human	IHC	J Mater Chem B; Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating; Authors - Bin Yan
human	IF	Nat Commun; Annexin A1 is a polarity cue that directs mitotic spindle orientation during mammalian epithelial morphogenesis; Authors - Maria Fankhaenel
mouse		J Proteome Res; Effect of Tamoxifen on Proteome Expression during In Vitro Myogenesis in Murine Skeletal Muscle C2C12 Cells; Authors - Emily A Morris
human	IF	Nature; Stress granules plug and stabilize damaged endolysosomal membranes; Authors - Claudio Bussi