AP1M1 Polyklonaler Antikörper

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• KD/KO Validated

AP1M1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 12112-1-AP

Synonyme

AP 1 complex subunit mu 1, AP mu chain family member mu1A, AP1M1, AP47, CLAPM2, CLTNM, MU 1A, Mu adaptin 1, Mu1A adaptin

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  WB analysis of Hacat cells using 12112-1-AP

  WB resut of AP1M1 antibody (12112-1-AP, 1:500) with siRNA cell lysate.

• 

  WB analysis of human brain using 12112-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12112-1-AP (AP1M1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 12112-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12112-1-AP (AP1M1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 12112-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12112-1-AP (AP1M1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 12112-1-AP

  IP result of anti-AP1M1 (IP:12112-1-AP, 4ug; Detection:12112-1-AP 1:500) with SH-SY5Y cells lysate 2500ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HaCaT-Zellen
• Erfolgreiche IP: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

12112-1-AP bindet in WB, IF, IP, CoIP, ELISA AP1M1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AP1M1 fusion protein Ag2757
• Vollständiger Name: adaptor-related protein complex 1, mu 1 subunit
• Berechnetes Molekulargewicht: 423 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC017469
• Gene symbol: AP1M1
• Gene ID (NCBI): 8907
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AP1M1, also named as CLTNM, Mu-adaptin 1 and Clathrin coat assembly protein AP47, belongs to the adaptor complexes medium subunit family. It is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; Plasma membrane contributes to the formation of pre-autophagosomal structures.; Authors - Ravikumar Brinda B ; KD Validated
human	IF,CoIP	Int J Mol Sci; Leucine Zipper Downregulated in Cancer-1 Interacts with Clathrin Adaptors to Control Epidermal Growth Factor Receptor (EGFR) Internalization and Gefitinib Response in EGFR-Mutated Non-Small Cell Lung Cancer; Authors - Hsien-Neng Huang
human	WB,IF	Traffic; AP-1A controls secretory granule biogenesis and trafficking of membrane secretory granule proteins.; Authors - Mathilde Bonnemaison ; KD Validated
	WB	J Biol Chem; Adaptor Protein-1 Complex Affects the Endocytic Trafficking and Function of Peptidylglycine α-Amidating Monooxygenase, a Luminal Cuproenzyme.; Authors - Mathilde L Bonnemaison ; KD Validated
human,mouse	WB	Biomed Pharmacother; Dioscin alleviates Alzheimer's disease through regulating RAGE/NOX4 mediated oxidative stress and inflammation.; Authors - Linshu Guan
	WB	J Biol Chem; Clathrin adaptor AP-1-mediated Golgi export of amyloid precursor protein is crucial for the production of neurotoxic amyloid fragments.; Authors - Yunan C Januário