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APEX1 Polyklonaler Antikörper

APEX1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(29)

Kat-Nr. : 10203-1-AP

Synonyme

APE, APE1, APE-1, APEN, APEX



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, HuH-7-Zellen, Maushirngewebe
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Magenkrebsgewebe, humanes Schilddrüsenkarzinomgewebe, Maus-Kolongewebe, Ratten-Kolongewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCMCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:1000-1:4000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

10203-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA APEX1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen APEX1 fusion protein Ag0251
Vollständiger Name APEX nuclease (multifunctional DNA repair enzyme) 1
Berechnetes Molekulargewicht 36 kDa
Beobachtetes Molekulargewicht 36 kDa
GenBank-ZugangsnummerBC002338
Gene symbol APEX1
Gene ID (NCBI) 328
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

APEX1, also named as APE, APE1, HAP1 and REF-1, belongs to the DNA repair enzymes AP/ExoA family. It is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 are in DNA repair and redox regulation of transcriptional factors. APEX nuclease is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. APEX1 is involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). When acetylated at Lys-6 and Lys-7, APEX1 stimulates the YBX1-mediated MDR1 promoter activity, leading to drug resistance. It also acts as an endoribonuclease involved in the control of single-stranded RNA metabolism. It plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, APEX1 plays a role in the rRNA quality control process during cell cycle progression. 10203-1-AP is a rabbit polyclonal antibody raised against full length APE1 of human origin.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for APEX1 antibody 10203-1-APProtokoll herunterladen
IHC protocol for APEX1 antibody 10203-1-APProtokoll herunterladenl
IF protocol for APEX1 antibody 10203-1-APProtokoll herunterladen
IP protocol for APEX1 antibody 10203-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Sci Adv

Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair

Authors - Dinh Duc Nguyen
mouseWB

Cell Death Dis

DNA polymerase beta connects tumorigenicity with the circadian clock in liver cancer through the epigenetic demethylation of Per1

Authors - Siyu Chen
humanWB,IHC

Mol Ther

miR-1293, a Candidate for miRNA-Based Cancer Therapeutics, Simultaneously Targets BRD4 and the DNA Repair Pathway.

Authors - Yuki Takagawa
  • KD Validated
ratIF

Free Radic Biol Med

Systematical exploration of transcription factors in cardiomyocytes against hydrogen peroxide-induced oxidative stress by Danhong injection.

Authors - Jingjing Zhang
humanWB,IP

Int Immunopharmacol

Inhibitors of APE1 redox and ATM synergistically sensitize osteosarcoma cells to ionizing radiation by inducing ferroptosis

Authors - Hanxi Xiao
  • KD Validated
humanWB

J Ethnopharmacol

Integrated network pharmacology, proteomics, molecular docking, and experiments in vivo and in vitro to explore the efficacy and potential mechanism of bufalin against hepatocellular carcinoma angiogenesis

Authors - Yuanchao Li
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