- Featured Product
- KD/KO Validated
APEX1 Polyklonaler Antikörper
APEX1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 10203-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HeLa-Zellen, HuH-7-Zellen, Maushirngewebe |
Erfolgreiche IP | HeLa-Zellen |
Erfolgreiche Detektion in IHC | humanes Magenkrebsgewebe, humanes Schilddrüsenkarzinomgewebe, Maus-Kolongewebe, Ratten-Kolongewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | MCF-7-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:1000-1:4000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 8 publications below |
WB | See 22 publications below |
IHC | See 5 publications below |
IF | See 4 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
ChIP | See 1 publications below |
Produktinformation
10203-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA APEX1 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | APEX1 fusion protein Ag0251 |
Vollständiger Name | APEX nuclease (multifunctional DNA repair enzyme) 1 |
Berechnetes Molekulargewicht | 36 kDa |
Beobachtetes Molekulargewicht | 36 kDa |
GenBank-Zugangsnummer | BC002338 |
Gene symbol | APEX1 |
Gene ID (NCBI) | 328 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
APEX1, also named as APE, APE1, HAP1 and REF-1, belongs to the DNA repair enzymes AP/ExoA family. It is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 are in DNA repair and redox regulation of transcriptional factors. APEX nuclease is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. APEX1 is involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). When acetylated at Lys-6 and Lys-7, APEX1 stimulates the YBX1-mediated MDR1 promoter activity, leading to drug resistance. It also acts as an endoribonuclease involved in the control of single-stranded RNA metabolism. It plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, APEX1 plays a role in the rRNA quality control process during cell cycle progression. 10203-1-AP is a rabbit polyclonal antibody raised against full length APE1 of human origin.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
---|---|
WB protocol for APEX1 antibody 10203-1-AP | Protokoll herunterladen |
IHC protocol for APEX1 antibody 10203-1-AP | Protokoll herunterladenl |
IF protocol for APEX1 antibody 10203-1-AP | Protokoll herunterladen |
IP protocol for APEX1 antibody 10203-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
---|---|
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Sci Adv Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair | ||
Cell Death Dis DNA polymerase beta connects tumorigenicity with the circadian clock in liver cancer through the epigenetic demethylation of Per1 | ||
Mol Ther miR-1293, a Candidate for miRNA-Based Cancer Therapeutics, Simultaneously Targets BRD4 and the DNA Repair Pathway.
| ||
Free Radic Biol Med Systematical exploration of transcription factors in cardiomyocytes against hydrogen peroxide-induced oxidative stress by Danhong injection. | ||
Int Immunopharmacol Inhibitors of APE1 redox and ATM synergistically sensitize osteosarcoma cells to ionizing radiation by inducing ferroptosis
| ||
J Ethnopharmacol Integrated network pharmacology, proteomics, molecular docking, and experiments in vivo and in vitro to explore the efficacy and potential mechanism of bufalin against hepatocellular carcinoma angiogenesis |