APLP2 Polyklonaler Antikörper

APLP2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA, PLA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 15041-1-AP

Synonyme

APLP-2, Amyloid-like protein 2, Amyloid like protein 2, Amyloid beta precursor like protein 2, Amyloid beta

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  WB analysis using 15041-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15041-1-AP (APLP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15041-1-AP

  IP result of anti-APLP2 (IP:15041-1-AP, 4ug; Detection:15041-1-AP 1:1000) with mouse brain tissue lysate 2100 ug.

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  IHC staining of mouse brain using 15041-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15041-1-AP (APLP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15041-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15041-1-AP (APLP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

15041-1-AP bindet in WB, IHC, IP, ELISA, PLA APLP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: APLP2 fusion protein Ag6838
• Vollständiger Name: amyloid beta (A4) precursor-like protein 2
• Berechnetes Molekulargewicht: 87 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC000373
• Gene symbol: APLP2
• Gene ID (NCBI): 334
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Amyloid precursor-like protein-2 (APLP2) is a member of the APP (amyloid precursor protein) family including APP, APLP1, and APLP2. APLP2 is ubiquitously expressed. It contains heparin-, copper- and zinc- binding domains at the N-terminus, BPTI/Kunitz inhibitor and E2 domains in the middle region, and transmembrane and intracellular domains at the C-terminus. APLP2 has been shown to regulate multiple cellular functions such as neurite outgrowth, axogenesis, corneal epithelial wound healing, cell adhesion, migration, and mitosis. The synergy of this protein and the APP is required to mediate neuromuscular transmission, spatial learning and synaptic plasticity. APLP2 has been implicated in the pathogenesis of Alzheimer's disease.

Publikationen

Species	Application	Title
		iScience; Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia; Authors - Yu-Han H Hsu
human	WB,PLA	Life Sci Alliance; Quantitative proteomics identifies PTP1B as modulator of B cell antigen receptor signaling.; Authors - Jennifer J Schwarz
human,mouse	WB	Heliyon; HMGA2 overexpression activates IGF2BP2 to stabilize APLP2 via m6A modification and promote pancreatic cancer progression; Authors - Ke Liu
		J Biol Chem; Familial Alzheimer's disease mutations in amyloid precursor protein impair calcineurin signaling to NMDA receptors; Authors - Steven J Tavalin