Apolipoprotein AI Monoklonaler Antikörper

Apolipoprotein AI Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(12)

CloneNo.

1C9G5

Kat-Nr. : 66206-1-Ig

Synonyme

APOA1, apolipoprotein A I, 1C9G5, APOA 1, ApoA I

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  WB analysis of human plasma using 66206-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66206-1-Ig (APOA1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66206-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66206-1-Ig (APOA1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66206-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66206-1-Ig (APOA1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 66206-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66206-1-Ig (APOA1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 66206-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66206-1-Ig (APOA1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66206-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66206-1-Ig (APOA1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HepG2 using 66206-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Apolipoprotein AI (66206-1-Ig, Clone:1C9G5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IHC: See 1 publications below

Produktinformation

66206-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Apolipoprotein AI und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Apolipoprotein AI fusion protein Ag21920
• Vollständiger Name: apolipoprotein A-I
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 26-28 kDa
• GenBank-Zugangsnummer: BC005380
• Gene symbol: APOA1
• Gene ID (NCBI): 335
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

Publikationen

Species	Application	Title
human	WB	J Extracell Vesicles; Optimized AF4 combined with density cushion ultracentrifugation enables profiling of high-purity human blood extracellular vesicles; Authors - Liqiao Hu
mouse	WB	EMBO J; Rab2A-mediated Golgi-lipid droplet interactions support very-low-density lipoprotein secretion in hepatocytes; Authors - Min Xu
human	WB	Elife; A critical role of VMP1 in lipoprotein secretion.; Authors - Hideaki Morishita
mouse	WB	Mol Cell Proteomics; Differential proteomic analysis of gender-dependent hepatic tumorigenesis in Hras12V transgenic mice.; Authors - Zhuona Rong
rat,human	WB	Peptides; Neuropeptide Y promotes hepatic apolipoprotein A1 synthesis and secretion through neuropeptide Y Y5 receptor.; Authors - Bingyang Liu
human	IHC	Mol Biol Rep; Differential proteomic profiles of exosomes in pediatric and adult adamantinomatous craniopharyngioma cyst fluid; Authors - Yiguang Chen