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APOE Monoklonaler Antikörper

APOE Monoklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(14)

CloneNo.

1B2C9

Kat-Nr. : 66830-1-Ig

Synonyme

1B2C9, Apo E, Apo-E, apoe4, apolipoprotein E



Geprüfte Anwendungen

Erfolgreiche Detektion in WBhumanes Plasmagewebe, Caco-2-Zellen, HepG2-Zellen, T-47D-Zellen
Erfolgreiche Detektion in IF/ICCHepG2-Zellen
Erfolgreiche Detektion in FC (Intra)HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:3000-1:20000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:400-1:1600
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66830-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), ELISA APOE und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen APOE fusion protein Ag28186
Vollständiger Name Apolipoprotein E
Berechnetes Molekulargewicht 36 kDa
Beobachtetes Molekulargewicht 34-36 kDa
GenBank-ZugangsnummerBC003557
Gene symbol APOE
Gene ID (NCBI) 348
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serve as a ligand for the LDL (APO B/E) receptor and for the specific APOE receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for APOE antibody 66830-1-IgProtokoll herunterladen
IF protocol for APOE antibody 66830-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
human,mouseIF

Nat Aging

Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer

Authors - Yifei Cheng
mouseWB

Brain Behav Immun

Egln3 expression in microglia enhances the neuroinflammatory responses in Alzheimer's disease

Authors - Jiaxin Guan
mouseWB

EMBO J

Rab2A-mediated Golgi-lipid droplet interactions support very-low-density lipoprotein secretion in hepatocytes

Authors - Min Xu
humanWB,IF

Elife

A critical role of VMP1 in lipoprotein secretion.

Authors - Hideaki Morishita
mouseIF

J Biol Chem

Lack of ApoE inhibits ADan amyloidosis in a mouse model of Familial Danish Dementia

Authors - Anllely Fernandez
mouseWB

Front Immunol

Binding of Macrophage Receptor MARCO, LDL, and LDLR to Disease-Associated Crystalline Structures.

Authors - Anika Alberts

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Silvia (Verified Customer) (12-03-2019)

Specific antibody for IF and WB on Human tissues

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: WB 1:3000, IF 1:500
  • Cell Tissue Type: Human organoids