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APOE Monoklonaler Antikörper
APOE Monoklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human und mehr (1)
Anwendung
WB, IHC, IF/ICC, FC (Intra), ELISA
Konjugation
Unkonjugiert
CloneNo.
1B2C9
Kat-Nr. : 66830-1-Ig
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | humanes Plasmagewebe, Caco-2-Zellen, HepG2-Zellen, T-47D-Zellen |
Erfolgreiche Detektion in IF/ICC | HepG2-Zellen |
Erfolgreiche Detektion in FC (Intra) | HepG2-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:3000-1:20000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:400-1:1600 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 10 publications below |
IHC | See 2 publications below |
IF | See 6 publications below |
Produktinformation
66830-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), ELISA APOE und zeigt Reaktivität mit human
Getestete Reaktivität | human |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Maus / IgG1 |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | APOE fusion protein Ag28186 |
Vollständiger Name | Apolipoprotein E |
Berechnetes Molekulargewicht | 36 kDa |
Beobachtetes Molekulargewicht | 34-36 kDa |
GenBank-Zugangsnummer | BC003557 |
Gene symbol | APOE |
Gene ID (NCBI) | 348 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-G-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serve as a ligand for the LDL (APO B/E) receptor and for the specific APOE receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for APOE antibody 66830-1-Ig | Protokoll herunterladen |
IF protocol for APOE antibody 66830-1-Ig | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Nat Aging Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer | ||
Brain Behav Immun Egln3 expression in microglia enhances the neuroinflammatory responses in Alzheimer's disease | ||
EMBO J Rab2A-mediated Golgi-lipid droplet interactions support very-low-density lipoprotein secretion in hepatocytes | ||
J Biol Chem Lack of ApoE inhibits ADan amyloidosis in a mouse model of Familial Danish Dementia | ||
Front Immunol Binding of Macrophage Receptor MARCO, LDL, and LDLR to Disease-Associated Crystalline Structures. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Silvia (Verified Customer) (12-03-2019) | Specific antibody for IF and WB on Human tissues
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