APOE Monoklonaler Antikörper

APOE Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

CloneNo.

1B2C9

Rezensionen (1)

Kat-Nr. : 66830-1-Ig

Synonyme

AD2, Apo E, APOE, apolipoprotein E, LPG

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Galerie der Validierungsdaten

• 

  WB analysis of human plasma using 66830-1-Ig

  human plasma was subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at a range of dilutions from 1:5000 to 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66830-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66830-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at dilution of 1:3400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human blood using 66830-1-Ig

  human blood was subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 66830-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66830-1-Ig (APOE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66830-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOE antibody (66830-1-Ig, Clone: 1B2C9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 66830-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human APOE (66830-1-Ig, Clone:1B2C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe, Caco-2-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:3000-1:20000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:400-1:1600
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Produktinformation

66830-1-Ig bindet in WB, IHC, IF, FC, ELISA APOE und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: APOE fusion protein Ag28186
• Vollständiger Name: apolipoprotein E
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 34-36 kDa
• GenBank-Zugangsnummer: BC003557
• Gene symbol: APOE
• Gene ID (NCBI): 348
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serve as a ligand for the LDL (APO B/E) receptor and for the specific APOE receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.

Publikationen

Species	Application	Title
human	WB,IF	Elife; A critical role of VMP1 in lipoprotein secretion.; Authors - Hideaki Morishita
mouse	IF	J Biol Chem; Lack of ApoE inhibits ADan amyloidosis in a mouse model of Familial Danish Dementia; Authors - Anllely Fernandez
mouse	WB	Front Immunol; Binding of Macrophage Receptor MARCO, LDL, and LDLR to Disease-Associated Crystalline Structures.; Authors - Anika Alberts
human	IHC,IF	Mol Oncol; Single-cell and spatial analyses reveal the association between gene expression of glutamine synthetase with the immunosuppressive phenotype of APOE+CTSZ+TAM in cancers; Authors - Jinfen Wei
mouse	WB,IF	Nat Neurosci; Lipid-accumulated reactive astrocytes promote disease progression in epilepsy; Authors - Zhang-Peng Chen
human	WB	Cancer Sci; Discovery of lipid profiles in plasma-derived extracellular vesicles as biomarkers for breast cancer diagnosis; Authors - Lin Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Silvia (Verified Customer) (12-03-2019)

Specific antibody for IF and WB on Human tissues

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: WB 1:3000, IF 1:500
• Cell Tissue Type: Human organoids

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C