APOE Rekombinanter Antikörper

APOE Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

242310B3

Kat-Nr. : 84775-6-RR

Synonyme

ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E

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  WB analysis using 84775-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84775-6-RR (APOE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using 84775-6-RR

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug APOE Recombinant antibody (84775-6-RR, Clone:242310B3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84775-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84775-6-RR against Human APOE were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HuH-7-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84775-6-RR bindet in WB, FC (Intra), ELISA APOE und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: Apolipoprotein E
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 34-38 kDa
• GenBank-Zugangsnummer: BC003557
• Gene symbol: APOE
• Gene ID (NCBI): 348
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serves as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight (PMID: 9831633).