APOL1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

APOL1 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

23

Rezensionen (1)

Kat-Nr. : 11486-2-AP

Synonyme

APO L, APOL, APOL I, APOL1, Apolipoprotein L, Apolipoprotein L I, apolipoprotein L, 1, Apolipoprotein L1

---

Galerie der Validierungsdaten

• 

  WB analysis of HepG2 using 11486-2-AP

  WB result of APOL1 antibody (11486-2-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-APOL1 transfected HepG2 cells.

• 

  WB analysis of human plasma using 11486-2-AP

  human blood were subjected to SDS PAGE followed by western blot with 11486-2-AP (APOL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11486-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11486-2-AP (APOL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11486-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11486-2-AP (APOL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 11486-2-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 11486-2-AP (APOL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SW 1990 using 11486-2-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 11486-2-AP (APOL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11486-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11486-2-AP (APOL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 11486-2-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11486-2-AP (APOL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11486-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11486-2-AP (APOL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11486-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11486-2-AP (APOL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11486-2-AP

  IP Result of anti-APOL1 (IP:11486-2-AP, 3ug; Detection:11486-2-AP 1:500) with HepG2 cells lysate 6000ug.

• 

  IP experiment of human plasma using 11486-2-AP

  IP Result of anti-APOL1 (IP:11486-2-AP, 4ug; Detection:11486-2-AP 1:500) with human plasma lysate 4000ug.

• 

  IHC staining of human kidney using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11486-2-AP (APOL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11486-2-AP (APOL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11486-2-AP (APOL1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11486-2-AP (APOL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11486-2-AP (APOL1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11486-2-AP (APOL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11486-2-AP (APOL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11486-2-AP (APOL1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11486-2-AP (APOL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11486-2-AP (APOL1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 11486-2-AP (APOL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11486-2-AP (APOL1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 11486-2-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11486-2-AP (APOL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11486-2-AP

  Immunofluorescent analysis of () fixed HepG2 cells using 11486-2-AP (APOL1 antibody) at dilution of 1:25.

• 

  IF Staining of HEK-293 using 11486-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using APOL1 antibody (11486-2-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 11486-2-AP

  1X10^6 HepG2 cells were stained with 0.2ug APOL1 antibody (11486-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, A2780-Zellen, A549-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, SKOV-3-Zellen, SW 1990-Zellen
• Erfolgreiche IP: HepG2-Zellen, humanes Plasmagewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Hodengewebe, humanes Lebergewebe, humanes Milzgewebe, humanes Pankreasgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:4000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Produktinformation

11486-2-AP bindet in WB, IP, IHC, IF, FC, ELISA APOL1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: APOL1 fusion protein Ag2016
• Vollständiger Name: apolipoprotein L, 1
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC017331
• Gene symbol: APOL1
• Gene ID (NCBI): 8542
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody recognizes the endogenous APOL1 of 39-45 kDa in blood lysate.

Publikationen

Species	Application	Title
human	WB	J Exp Med; Distinct roles of apolipoprotein components within the trypanosome lytic factor complex revealed in a novel transgenic mouse model.; Authors - Molina-Portela Maria Pilar MP
human	WB	Proc Natl Acad Sci U S A; Hydrodynamic gene delivery of baboon trypanosome lytic factor eliminates both animal and human-infective African trypanosomes.; Authors - Thomson Russell R
	WB	Proc Natl Acad Sci U S A; Evolution of the primate trypanolytic factor APOL1.; Authors - Russell Thomson
human	WB	J Am Soc Nephrol; Domain-Specific Antibodies Reveal Differences in the Membrane Topologies of Apolipoprotein L1 in Serum and Podocytes.; Authors - Nidhi Gupta ; KD Validated
human	WB,IF	J Am Soc Nephrol; Apolipoprotein L1-Specific Antibodies Detect Endogenous APOL1 inside the Endoplasmic Reticulum and on the Plasma Membrane of Podocytes.; Authors - Suzie J Scales ; KO Validated
human	IHC	Br J Cancer; A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.; Authors - Xiaohui Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

David (Verified Customer) (02-04-2023)

Good antibody for detect apol1 protein

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 to 200
• Cell Tissue Type: 293T

APOL1 Antibody Immunofluorescence validation (1 to 200 dilution) in 293T (Cat no:11486-2-AP)

Verwandte Produkte

APOL1-Specific Monoclonal antibody

66124-1-Ig

MYH9 Polyclonal antibody

11128-1-AP

MYH9 Monoclonal antibody

60233-1-Ig

Apolipoprotein AI Polyclonal antibody

14427-1-AP

Apolipoprotein AI Monoclonal antibody

66206-1-Ig