APOL1-Specific Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

APOL1-Specific Monoklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

15

CloneNo.

1G12D11

Rezensionen (1)

Kat-Nr. : 66124-1-Ig

Synonyme

APO L, APOL, APOL I, APOL1, APOL1-Specific, Apolipoprotein L, Apolipoprotein L I, apolipoprotein L, 1, Apolipoprotein L1

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Galerie der Validierungsdaten

• 

  WB analysis of human plasma using 66124-1-Ig

  1.5 ug human plasma was subjected to SDS PAGE followed by western blot with 66124-1-Ig (APOL1 Antibody) at dilution of 1:400, 000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human plasma using 66124-1-Ig

  IP Result of anti-APOL1 (IP:66124-1-Ig, 5ug; Detection:66124-1-Ig 1:10000) with human plasma lysate 4000ug.

• 

  IHC staining of human liver using 66124-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66124-1-Ig(APOL1 antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human kidney using 66124-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66124-1-Ig (APOL1-Specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66124-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66124-1-Ig (APOL1-Specific antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66124-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66124-1-Ig(APOL1 antibody) at dilution of 1:500 (under 40x lens).

• 

  IHC staining of human kidney using 66124-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 66124-1-Ig(APOL1 antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human kidney using 66124-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney using 66124-1-Ig(APOL1 antibody) at dilution of 1:500 (under 40x lens).

• 

  IHC staining of human liver using 66124-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66124-1-Ig(APOL1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 66124-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66124-1-Ig(APOL1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HepG2 using 66124-1-Ig

  1X10^6 HepG2 cells were stained with 0.2ug APOL1 antibody (66124-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe
• Erfolgreiche IP: humanes Plasmagewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:5000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Produktinformation

66124-1-Ig bindet in WB, IP, IHC, IF, FC, ELISA APOL1-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: APOL1-Specific fusion protein Ag2016
• Vollständiger Name: apolipoprotein L, 1
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 39-45 kDa
• GenBank-Zugangsnummer: BC017331
• Gene symbol: APOL1
• Gene ID (NCBI): 8542
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody recognizes the endogenous ApoL1 of 39-45 kDa in blood lysate. This antibody is specific to APOL1.

Publikationen

Species	Application	Title
	WB,IF	Immunity; APOL1 risk variants in individuals of African genetic ancestry drive endothelial cell defects that exacerbate sepsis.; Authors - Junnan Wu
	WB	J Clin Invest; The key role of NLRP3 and STING in APOL1-associated podocytopathy.; Authors - Junnan Wu
human	WB	J Am Soc Nephrol; Kidney Disease-Associated APOL1 Variants Have Dose-Dependent, Dominant Toxic Gain-of-Function.; Authors - Somenath Datta
	WB	Mol Ther; Antisense oligonucleotides ameliorate kidney dysfunction in podocyte-specific APOL1 risk variant mice.; Authors - Ya-Wen Yang
human	WB,IF	Elife; Apolipoprotein L-1 renal risk variants form active channels at the plasma membrane driving cytotoxicity.; Authors - Joseph A Giovinazzo
human	WB,IHC	Neurobiol Dis; Apolipoprotein L1 is increased in frontotemporal lobar degeneration post-mortem brain but not in ante-mortem cerebrospinal fluid.; Authors - Yanaika S Hok-A-Hin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jonathan (Verified Customer) (11-11-2019)

Works well for WB.

• Applications: Western Blot,

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