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APOL1-Specific Monoklonaler Antikörper

APOL1-Specific Monoklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

CloneNo.

1G12D11

Kat-Nr. : 66124-1-Ig

Synonyme

APOL1, 1G12D11, Apo-L, ApoL-I, Apolipoprotein L I



Geprüfte Anwendungen

Erfolgreiche Detektion in WBhumanes Plasmagewebe
Erfolgreiche IPhumanes Plasmagewebe
Erfolgreiche Detektion in IHChumanes Lebergewebe, humanes Nierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:20000-1:100000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:500-1:5000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66124-1-Ig bindet in WB, IHC, IF/ICC, IP, ELISA APOL1-Specific und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen APOL1-Specific fusion protein Ag2016
Vollständiger Name apolipoprotein L, 1
Berechnetes Molekulargewicht 44 kDa
Beobachtetes Molekulargewicht 39-45 kDa
GenBank-ZugangsnummerBC017331
Gene symbol APOL1
Gene ID (NCBI) 8542
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody recognizes the endogenous ApoL1 of 39-45 kDa in blood lysate. This antibody is specific to APOL1.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for APOL1-Specific antibody 66124-1-IgProtokoll herunterladen
IHC protocol for APOL1-Specific antibody 66124-1-IgProtokoll herunterladenl
IF protocol for APOL1-Specific antibody 66124-1-IgProtokoll herunterladen
IP protocol for APOL1-Specific antibody 66124-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
WB,IF

Immunity

APOL1 risk variants in individuals of African genetic ancestry drive endothelial cell defects that exacerbate sepsis.

Authors - Junnan Wu
WB

J Clin Invest

The key role of NLRP3 and STING in APOL1-associated podocytopathy.

Authors - Junnan Wu
humanWB

J Am Soc Nephrol

Kidney Disease-Associated APOL1 Variants Have Dose-Dependent, Dominant Toxic Gain-of-Function.

Authors - Somenath Datta
WB

Mol Ther

Antisense oligonucleotides ameliorate kidney dysfunction in podocyte-specific APOL1 risk variant mice.

Authors - Ya-Wen Yang
humanWB,IF

Elife

Apolipoprotein L-1 renal risk variants form active channels at the plasma membrane driving cytotoxicity.

Authors - Joseph A Giovinazzo
humanWB

Biomolecules

APOL1 Modulates Renin-Angiotensin System

Authors - Vinod Kumar

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Jonathan (Verified Customer) (11-11-2019)

Works well for WB.

  • Applications: Western Blot,