AQP1 Polyklonaler Antikörper

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• KD/KO Validated

AQP1 Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (4)

Anwendung

WB, IHC, IF-P, IF-Fro, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(79)

Kat-Nr. : 20333-1-AP

Synonyme

Aquaporin 1, AQP 1, AQP-1, Aquaporin1, Aquaporin-1

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  WB analysis using 20333-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20333-1-AP (AQP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20333-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20333-1-AP (AQP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 20333-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 20333-1-AP (AQP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 20333-1-AP

  IP result of anti-AQP1 (IP:20333-1-AP, 4ug; Detection:20333-1-AP 1:40000) with mouse kidney tissue lysate 2160 ug.

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  IP experiment of mouse skeletal muscle using 20333-1-AP

  IP result of anti-AQP1 (IP:20333-1-AP, 4ug; Detection:20333-1-AP 1:1500) with mouse skeletal muscle tissue lysate 3600ug.

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  IHC staining of human breast cancer using 20333-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20333-1-AP (AQP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20333-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20333-1-AP (AQP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 20333-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20333-1-AP (AQP1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 20333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20333-1-AP (AQP1 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 20333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20333-1-AP (AQP1 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human breast cancer using 20333-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using AQP1 antibody (20333-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse kidney using 20333-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using AQP1 antibody (20333-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse kidney using 20333-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using AQP1 antibody (20333-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse breast cancer using 20333-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using AQP1 antibody (20333-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, humanes Herzgewebe
• Erfolgreiche IP: Mausnierengewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Mammakarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Mammakarzinomgewebe, Mausnierengewebe
• Erfolgreiche Detektion in IF-Fro: mouse breast cancer

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:3000-1:12000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 37 publications below
• IHC: See 27 publications below
• IF: See 29 publications below
• IP: See 2 publications below
• FC: See 2 publications below

Produktinformation

20333-1-AP bindet in WB, IHC, IF-P, IF-Fro, IP, ELISA AQP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Maus, Ratte, Rind, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AQP1 fusion protein Ag14093
• Vollständiger Name: aquaporin 1 (Colton blood group)
• Berechnetes Molekulargewicht: 269 aa, 29 kDa
• Beobachtetes Molekulargewicht: 25-28 kDa, 35-50 kDa
• GenBank-Zugangsnummer: BC022486
• Gene symbol: AQP1
• Gene ID (NCBI): 358
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 35-50 kDa. (PMID:20965731,16508653, 1530176).

Publikationen

Species	Application	Title
pig	IHC	Kidney Int; Physiologic Homeostasis after Pig-to-Human Kidney Xenotransplantation; Authors - Eric Judd
human	WB	J Extracell Vesicles; Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease; Authors - Rong Yang
human	IF	Theranostics; Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI; Authors - Bojun Li
human	IHC	Proc Natl Acad Sci U S A; Direct visualization of the arterial wall water permeability barrier using CARS microscopy.; Authors - Bertrand M Lucotte
human	IHC,IF	Cancer Res; Stromal-Based Signatures for the Classification of Gastric Cancer.; Authors - Mark T Uhlik
	WB,IF	Cell Rep; Multicilia dynamically transduce Sonic Hedgehog signaling to regulate choroid plexus functions; Authors - Suifang Mao