AQP2 Polyklonaler Antikörper

AQP2 Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, IF-Fro, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 29386-1-AP

Synonyme

Aquaporin 2, AQP-2, Aquaporin2

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  WB analysis of mouse kidney using 29386-1-AP

  37°C incubated mouse kidney tissue were subjected to SDS PAGE followed by western blot with 29386-1-AP (AQP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 29386-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29386-1-AP (AQP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 29386-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29386-1-AP (AQP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 29386-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using AQP2 antibody (29386-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), AQP1 antibody (66805-1-Ig, Clone: 2E5D6, green).

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  IF Staining of mouse kidney using 29386-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse kidney tissue using AQP2 antibody (29386-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: bei 37°C inkubiertes Mausnierengewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausnierengewebe
• Erfolgreiche Detektion in IF-Fro: Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Produktinformation

29386-1-AP bindet in WB, IHC, IF-P, IF-Fro, ELISA AQP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AQP2 fusion protein Ag29931
• Vollständiger Name: aquaporin 2 (collecting duct)
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 28-29, 35-38 kDa
• GenBank-Zugangsnummer: NM_000486
• Gene symbol: AQP2
• Gene ID (NCBI): 359
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AQP2 (Aquaporin2) is a water channel protein that is widely distributed among mammalian tissues and plays a major role in water homeostasis. AQP2 is mainly found in the apical cell membranes of the kidney's collecting duct cells and is critical for urinary concentration. AQP2 can be glycosylated and this antibody recognizes the 28-29 kDa non-glycosylated as well as the 35-40 kDa glycosylated forms of AQP2.

Publikationen

Species	Application	Title
mouse	WB,IF	FASEB J; Circadian rhythm disruptions exacerbate inner ear damage in a murine endolymphatic hydrops model; Authors - Bo Pang
mouse	IF	Environ Pollut; Ozone exposure induced kidney damage in diabetic mice: the key role of lipid metabolism and water-electrolyte homeostasis; Authors - Yaru Zhang
mouse	IF	Diabetes Metab J; Kidney Gastrin/CCKBR Attenuates Type 2 Diabetes Mellitus by Inhibiting SGLT2-Mediated Glucose Reabsorption through Erk/NF-κB Signaling Pathway; Authors - Xue Zhang
mouse	IF	Research (Wash D C); Single-Cell RNA Sequencing Analysis Reveals the Role of Macrophage-Mediated CD44-AKT-CCL2 Pathways in Renal Tubule Injury during Calcium Oxalate Crystal Formation; Authors - Xi Jin
mouse	IHC	EMBO Mol Med; Melanin-like nanoparticles slow cyst growth in ADPKD by dual inhibition of oxidative stress and CREB; Authors - Yongzhan Sun