Amphiregulin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Amphiregulin Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Kat-Nr. : 16036-1-AP

Synonyme

amphiregulin, AR, AREG, AREGB, CRDGF, SDGF

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 16036-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16036-1-AP (Amphiregulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 16036-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16036-1-AP (Amphiregulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16036-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16036-1-AP (Amphiregulin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16036-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16036-1-AP (Amphiregulin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 16036-1-AP

  IP Result of anti-Amphiregulin (IP:16036-1-AP, 4ug; Detection:16036-1-AP 1:2000) with HepG2 cells lysate 3600ug.

• 

  IHC staining of human colon cancer using 16036-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16036-1-AP (Amphiregulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16036-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16036-1-AP (Amphiregulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 16036-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16036-1-AP (Amphiregulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon cancer using 16036-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Amphiregulin antibody (16036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human colon cancer using 16036-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Amphiregulin antibody (16036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 16036-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Amphiregulin (16036-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, A549-Zellen, HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Kolonkarzinomgewebe
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IHC: See 5 publications below
• IF: See 5 publications below
• IP: See 2 publications below

Produktinformation

16036-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Amphiregulin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Amphiregulin fusion protein Ag8907
• Vollständiger Name: amphiregulin
• Berechnetes Molekulargewicht: 252 aa, 28 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC009799
• Gene symbol: AREG
• Gene ID (NCBI): 374
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Amphiregulin (AREG) is one of the ligands of the epidermal growth factor receptor (EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs. Post-translastional modfications of 50-kDa pro-AREG produces a major soluble 43-kDa form, 28-, 26-, 16-kDa membrane anchored forms, and soluble 21-, 19-, and 9-kDa forms (PMID: 9642297).

Publikationen

Species	Application	Title
human	WB	Cancer Res; CD317 Activates EGFR by Regulating Its Association with Lipid Rafts.; Authors - Guizhong Zhang
human	IP	Proc Natl Acad Sci U S A; AhR controls redox homeostasis and shapes the tumor microenvironment in BRCA1-associated breast cancer.; Authors - Shawn P Kubli
human	WB	Cell Biosci; TPM2 attenuates progression of prostate cancer by blocking PDLIM7-mediated nuclear translocation of YAP1; Authors - Zonglong Wu
human	WB, IF	Oncogene; The EGF receptor ligand amphiregulin controls cell division via FoxM1.; Authors - S W Stoll
human	WB,IHC,IP	Aging Cell; Targeting amphiregulin (AREG) derived from senescent stromal cells diminishes cancer resistance and averts programmed cell death 1 ligand (PD-L1)-mediated immunosuppression.; Authors - Qixia Xu ; KD Validated
human	WB,IF	J Invest Dermatol; Membrane-Tethered Intracellular Domain of Amphiregulin Promotes Keratinocyte Proliferation.; Authors - Stefan W Stoll

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