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ARF1 Polyklonaler Antikörper

ARF1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 20226-1-AP

Synonyme

ADP ribosylation factor 1, ADP-ribosylation factor 1, EC:3.6.5.2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBDU 145-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Maushirngewebe, SKOV-3-Zellen
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Kolonkarzinomgewebe, humanes Hirngewebe, humanes Lebergewebe, Mauslebergewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen
Erfolgreiche Detektion in FC (Intra)HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

20226-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA ARF1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name ADP-ribosylation factor 1
Berechnetes Molekulargewicht 21 kDa
Beobachtetes Molekulargewicht 18-21 kDa
GenBank-ZugangsnummerNM_001658
Gene symbol ARF1
Gene ID (NCBI) 375
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment.(PMID: 7759471, PMID: 16042562). The antibody is specific to ARF1.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for ARF1 antibody 20226-1-APProtokoll herunterladen
IHC protocol for ARF1 antibody 20226-1-APProtokoll herunterladenl
IF protocol for ARF1 antibody 20226-1-APProtokoll herunterladen
IP protocol for ARF1 antibody 20226-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Nat Immunol

Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.

Authors - Miwa Sasai
mouse,humanWB

Nat Commun

Myristic acid as a checkpoint to regulate STING-dependent autophagy and interferon responses by promoting N-myristoylation

Authors - Mutian Jia
humanWB

Nat Commun

ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling

Authors - Maximilian Hirschenberger
humanWB

PLoS Pathog

Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.

Authors - Tao Wang
humanWB,IF

J Cell Sci

The protein kinase D1-mediated classical protein secretory pathway regulates the Ras oncogene-induced senescence response.

Authors - Yuanyuan Su
humanWB

Sci Rep

In vivo monitoring of the recruitment and activation of AP-1 by Arf1.

Authors - Etienne Sauvageau

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Cristian (Verified Customer) (04-26-2019)

Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 20226-1-AP (ARF1 antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HeLa
ARF1 Antibody Immunofluorescence, validation (1:500 dilution) in HeLa (Cat no:20226-1-AP)