ARG2 Polyklonaler Antikörper

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• KD/KO Validated

ARG2 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 14825-1-AP

Synonyme

ARG2, Arginase 2, mitochondrial, arginase, type II, Kidney type arginase, Non hepatic arginase, Type II arginase

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Galerie der Validierungsdaten

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  WB analysis using 14825-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14825-1-AP (ARG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 14825-1-AP

  WB result of ARG2 antibody (14825-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARG2 transfected Caco-2 cells.

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  WB analysis of HEK-293 using 14825-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14825-1-AP (ARG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 14825-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14825-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14825-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 14825-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14825-1-AP (ARG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14825-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14825-1-AP (ARG2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HEK-293-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

14825-1-AP bindet in WB, IHC, IF, ELISA ARG2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARG2 fusion protein Ag6609
• Vollständiger Name: arginase, type II
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39-42 kDa
• GenBank-Zugangsnummer: BC001350
• Gene symbol: ARG2
• Gene ID (NCBI): 384
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARG2(Arginase-2, mitochondrial) is also named as kidney-type arginase, non-hepatic arginase and belongs to the arginase family. It may play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis.

Publikationen

Species	Application	Title
mouse	IF	Circulation; Deletion of Macrophage Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Accelerates Atherosclerosis Regression and Increases C-C Chemokine Receptor Type 7 (CCR7) Expression in Plaque Macrophages.; Authors - Paul A Mueller
mouse	WB	J Lipid Res; Macrophage-derived apoESendai suppresses atherosclerosis while causing lipoprotein glomerulopathy in hyperlipidemic mice.; Authors - Hagai Tavori
mouse	WB	Biomed Pharmacother; PMID: 30889486; Authors - Lu Gao
mouse	WB	Biochim Biophys Acta; Reduced bioavailable manganese causes striatal urea cycle pathology in Huntington's disease mouse model.; Authors - Terry Jo V Bichell
	IHC	Biol Reprod; Progesterone and interferon tau regulate expression of polyamine enzymes during the ovine peri-implantation period.; Authors - Katherine M Halloran
human	WB	Life Sci; Ornithine is a key mediator in hyperphosphatemia-mediated human umbilical vein endothelial cell apoptosis: Insights gained from metabolomics.; Authors - Rong Zhou