ARHGAP21 Polyklonaler Antikörper

ARHGAP21 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Rezensionen (1)

Kat-Nr. : 22183-1-AP

Synonyme

ARHGAP21, KIAA1424

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Galerie der Validierungsdaten

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  WB analysis using 22183-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22183-1-AP (ARHGAP21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary using 22183-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 22183-1-AP (ARHGAP21 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 22183-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22183-1-AP (ARHGAP21 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 22183-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22183-1-AP (ARHGAP21 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 22183-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 22183-1-AP (ARHGAP21 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 22183-1-AP

  Immunofluorescent analysis of HepG2 cells using 22183-1-AP (ARHGAP21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Eierstockgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

22183-1-AP bindet in WB, IHC, IF, ELISA ARHGAP21 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARHGAP21 fusion protein Ag16515
• Vollständiger Name: Rho GTPase activating protein 21
• Berechnetes Molekulargewicht: 1957 aa, 217 kDa
• Beobachtetes Molekulargewicht: 250 kDa
• GenBank-Zugangsnummer: BC118915
• Gene symbol: ARHGAP21
• Gene ID (NCBI): 57584
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Sci Adv; Phosphorylation-dependent recognition of diverse protein targets by the cryptic GK domain of MAGI MAGUKs; Authors - Meng Zhang ; KD Validated
human	WB,IF	Elife; PTEN controls glandular morphogenesis through a juxtamembrane β-Arrestin1/ARHGAP21 scaffolding complex.; Authors - Arman Javadi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joleen (Verified Customer) (06-03-2019)

first 2 lanes are whole cell lysate. ARHGAP21doesn't show up prominently but does show up in last 2 lanes which are purified biotinylated proteins surrounding out protein of interest. This seems to indicate that the antibody works but just that the cells we use express levels that are below the level of detection unless it is enriched (such as with our purification protocol)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

ARHGAP21 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:22183-1-AP)