GRAF Polyklonaler Antikörper
GRAF Polyklonal Antikörper für WB, IHC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 17747-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HeLa-Zellen, HepG2-Zellen, humanes Hirngewebe, Maus-Thymusgewebe |
Erfolgreiche IP | HepG2-Zellen |
Erfolgreiche Detektion in IHC | Maushirngewebe, humanes Herzgewebe, humanes Lebergewebe, Rattenhirngewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 4 publications below |
IHC | See 2 publications below |
CoIP | See 1 publications below |
Produktinformation
17747-1-AP bindet in WB, IHC, IP, CoIP, ELISA GRAF und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | GRAF fusion protein Ag12008 |
Vollständiger Name | Rho GTPase activating protein 26 |
Berechnetes Molekulargewicht | 759 aa, 86 kDa |
Beobachtetes Molekulargewicht | 92 kDa |
GenBank-Zugangsnummer | BC068555 |
Gene symbol | ARHGAP26 |
Gene ID (NCBI) | 23092 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
GTPase Regulator Associated with Focal Adhesion Kinase (GRAF), also known as Rho GTPase activating protein 26 (ARHGAP26) , is a GTPase-activating protein and inhibits the activity of Rho GTPases by promoting the hydrolytic ability of Rho GTPases. GRAF enhances the hydrolysis of GTPases and converts GTPases from an active form to an inactive form, thereby inhibiting signaling transduction. Deletion and mutation of GRAF can lead to promyelocytic leukemia, suggesting tumor suppressive activity of GRAF. GRAF was downregulated in glioblastoma and associated with cell proliferation and migration (PMID: 10908648, PMID: 17611651, PMID: 31004081). It has been reported that there are three splicing mutants of GRAF, namely GRAF1a (92 kDa),GRAF1b (86 kDa) and GRAF1c (75-82 kDa), and GRAF1b and GRAF1c are the major GRAF1 isoforms in adult brain, whereas GRAF1a is abundant in neonates(PMID: 35624318, PMID: 30626696).
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for GRAF antibody 17747-1-AP | Protokoll herunterladen |
IHC protocol for GRAF antibody 17747-1-AP | Protokoll herunterladenl |
IP protocol for GRAF antibody 17747-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
J Neurol Rho GTPase-activating protein 10 (ARHGAP10/GRAF2) is a novel autoantibody target in patients with autoimmune encephalitis | ||
Brain Res Deficiency of NEAT1 prevented MPP+-induced inflammatory response, oxidative stress and apoptosis in dopaminergic SK-N-SH neuroblastoma cells via miR-1277-5p/ARHGAP26 axis. | ||
Cell Death Dis CEMIP, acting as a scaffold protein for bridging GRAF1 and MIB1, promotes colorectal cancer metastasis via activating CDC42/MAPK pathway | ||
World J Surg Oncol Prognostic value of circadian rhythm-associated genes in breast cancer |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Cicek (Verified Customer) (06-11-2024) | I used this antibody on mouse neurons, iPSCs and iNeurons with Western blotting, using beta-actin as positive control. There were specific bands at 90 kDa mark for iPSCs and iNeurons, but not for mouse neurons. It was highly expressed in iNeurons. There were unspecific bands at the 70 kDa mark for all samples.
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