ARHGDIB Monoklonaler Antikörper

ARHGDIB Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

3E7B7

Kat-Nr. : 66054-1-Ig

Synonyme

ARHGDIB, D4, GDIA2, GDID4, Ly GDI, LYGDI, RAP1GN1, Rho GDI 2, Rho GDI beta, RhoGDI2

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Galerie der Validierungsdaten

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  WB analysis using 66054-1-Ig

  Various lysates (25μg/lane) were subjected to SDS PAGE followed by western blot with 66054-1-Ig (ARHGDIB antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cerebellum using 66054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cerebellum using 66054-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66054-1-Ig (ARHGDIB antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 66054-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ARHGDIB antibody (66054-1-Ig, Clone: 3E7B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human tonsillitis using 66054-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ARHGDIB antibody (66054-1-Ig, Clone: 3E7B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, HL-60-Zellen, Jurkat-Zellen, Raji-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:10000-1:50000
• Immunhistochemie (IHC): IHC : 1:5000-1:10000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66054-1-Ig bindet in WB, IHC, IF, ELISA ARHGDIB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ARHGDIB fusion protein Ag9091
• Vollständiger Name: Rho GDP dissociation inhibitor (GDI) beta
• Berechnetes Molekulargewicht: 201 aa, 23 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC009200
• Gene symbol: ARHGDIB
• Gene ID (NCBI): 397
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARHGDIB, also known as RhoGDI2, is a conserved member of the RhoGDI family and plays an important role in cell migrations. It regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. It is mainly in hematopoietic, endothelial, and epithelial cells. It has been linked to tumorigenesis and metastasis. RhoGDI2 expression is downregulated in several cancer types, such as bladder, lung and lymphoma, but is upregulated in prostate and gastric cancer.

Publikationen

Species	Application	Title
human	WB	Hematology; Bioinformatic analysis of the expression profile and identification of RhoGDI2 as a biomarker in imatinib-resistant K562 cells; Authors - Yulin Yang