ARHGEF1 Polyklonaler Antikörper

ARHGEF1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (3)

Kat-Nr. : 11363-1-AP

Synonyme

ARHGEF1, GEF1, LBCL2, LSC, P115 RHOGEF, p115RhoGEF, SUB1.5

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 11363-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11363-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11363-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11363-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11363-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 11363-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 11363-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11363-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11363-1-AP (ARHGEF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11363-1-AP

  IP Result of anti-ARHGEF1 (IP:11363-1-AP, 4ug; Detection:11363-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

• 

  IHC staining of human colon using 11363-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 11363-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 11363-1-AP

  Immunofluorescent analysis of HeLa cells using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 11363-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Hirngewebe, humanes Milzgewebe, Jurkat-Zellen, K-562-Zellen, Mausmilzgewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

11363-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA ARHGEF1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARHGEF1 fusion protein Ag1905
• Vollständiger Name: Rho guanine nucleotide exchange factor (GEF) 1
• Berechnetes Molekulargewicht: 912 aa, 102 kDa
• Beobachtetes Molekulargewicht: 102-110 kDa
• GenBank-Zugangsnummer: BC011726
• Gene symbol: ARHGEF1
• Gene ID (NCBI): 9138
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nature; Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.; Authors - Silvana Konermann
human	WB	Nat Metab; Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression; Authors - Lin Qian
human	WB	Mol Biol Cell; The WASH complex, an endosomal Arp2/3 activator, interacts with the Hermansky-Pudlak syndrome complex BLOC-1 and its cargo phosphatidylinositol-4-kinase type IIα.; Authors - P V Ryder
mouse	CoIP,WB	Nat Commun; Integrin β3 directly inhibits the Gα13-p115RhoGEF interaction to regulate G protein signaling and platelet exocytosis; Authors - Yaping Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (12-29-2023)

Worked well for IP using 6ug Ab and WB 1:1500

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioma

FH

can (Verified Customer) (12-17-2019)

Very good antibody!

• Applications: Immunoprecipitation,
• Cell Tissue Type: blood vascular cell

FH

Ni (Verified Customer) (09-26-2019)

Very successful in immunoprecipitating p115RhoGEF from transfected cells.

• Applications: Immunoprecipitation,
• Primary Antibody Dilution: 2 ug /reaction