ARHGEF18 Polyklonaler Antikörper

ARHGEF18 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11243-1-AP

Synonyme

114 kDa Rho-specific guanine nucleotide exchange factor, KIAA0521, p114 Rho GEF, P114 RhoGEF, p114RhoGEF

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  WB analysis of mouse testis using 11243-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11243-1-AP (ARHGEF18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 11243-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11243-1-AP (ARHGEF18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 11243-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11243-1-AP (ARHGEF18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 11243-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11243-1-AP (ARHGEF18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11243-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11243-1-AP (ARHGEF18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11243-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11243-1-AP (ARHGEF18 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 11243-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11243-1-AP (ARHGEF18 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A431 using 11243-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ARHGEF18 antibody (11243-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Nierengewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11243-1-AP bindet in WB, IHC, IF/ICC, ELISA ARHGEF18 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARHGEF18 fusion protein Ag1749
• Vollständiger Name: rho/rac guanine nucleotide exchange factor (GEF) 18
• Berechnetes Molekulargewicht: 131 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: BC014994
• Gene symbol: ARHGEF18
• Gene ID (NCBI): 23370
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.