ARHGEF4 Polyklonaler Antikörper

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• KD/KO Validated

ARHGEF4 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 55213-1-AP

Synonyme

ARHGEF4, ASEF, ASEF1, GEF4, KIAA1112, STM6

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 55213-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55213-1-AP (ARHGEF4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of multi-cells/tissue using 55213-1-AP

  WB result of 55213-1-AP (ARHGEF4 Antibody).

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  IP experiment of mouse brain using 55213-1-AP

  IP Result of anti-ARHGEF4 (IP:55213-1-AP, 4ug; Detection:55213-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human brain using 55213-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55213-1-AP (ARHGEF4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 55213-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55213-1-AP (ARHGEF4 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Multizellen/-gewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

55213-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA ARHGEF4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Rho guanine nucleotide exchange factor (GEF) 4
• Berechnetes Molekulargewicht: 79 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: NM_015320
• Gene symbol: ARHGEF4
• Gene ID (NCBI): 50649
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARHGEF4, also named as KIAA1112 and Asef, acts as guanine nucleotide exchange factor (GEF) for RhoA and RAC1 GTPases. Binding of APC, ARHGEF4 may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion.(PMID: 10873612). This antibody is specific to ARHGEF4.

Publikationen

Species	Application	Title
human	CoIP	Nat Chem Biol; Peptidomimetic inhibitors of APC-Asef interaction block colorectal cancer migration.; Authors - Haiming Jiang
human,mouse	IHC,IF	J Neurosci; Temporal Profiling of Astrocyte Precursors Reveals Parallel Roles for Asef during Development and after Injury.; Authors - Lesley S Chaboub ; KO Validated
human	IHC	iScience; Whole-genome DNA methylation and DNA methylation-based biomarkers in lung squamous cell carcinoma; Authors - Qidong Cai
		Int J Oncol; ARHGEF4 predicts poor prognosis and promotes cell invasion by influencing ERK1/2 and GSK-3α/β signaling in pancreatic cancer.; Authors - Keisuke Taniuchi
mouse	WB	Biol Reprod; Raptor directs Sertoli cell cytoskeletal organization and polarity in the mouse testis.; Authors - Zhi Xiong
human	WB	Cell Adh Migr; ARHGEF4-mediates the actin cytoskeleton reorganization of hepatic stellate cells in 3-dimensional collagen matrices.; Authors - Xiaowei Zhang ; KD Validated