ARHGEF7 Polyklonaler Antikörper

ARHGEF7 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 14092-1-AP

Synonyme

KIAA0142, COOL-1, COOL1, COOL 1, Beta-Pix

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  WB analysis of PC-12 using 14092-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 14092-1-AP

  WB result of ARHGEF7 antibody (14092-1-AP; 1:1000; room temperature for 1.5 hours) with negative control and ARHGEF7 overexpressed Transfected HEK-293 cells.

• 

  WB analysis of K-562 using 14092-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 14092-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14092-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14092-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 14092-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14092-1-AP (ARHGEF7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14092-1-AP

  IP result of anti-ARHGEF7 (IP:14092-1-AP, 4ug; Detection:14092-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of mouse brain using 14092-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14092-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 14092-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 14092-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human rectal cancer using 14092-1-AP

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human rectal cancer using 14092-1-AP

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14092-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14092-1-AP (ARHGEF7 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Maus-Skelettmuskelgewebe, NIH/3T3-Zellen, Transfizierte HEK-293-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

14092-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ARHGEF7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARHGEF7 fusion protein Ag5223
• Vollständiger Name: Rho guanine nucleotide exchange factor (GEF) 7
• Berechnetes Molekulargewicht: 803 aa, 90 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC060776
• Gene symbol: ARHGEF7
• Gene ID (NCBI): 8874
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Phosphorylation of LRRK2 by casein kinase 1α regulates trans-Golgi clustering via differential interaction with ARHGEF7.; Authors - Ruth Chia
human	WB,IHC	Am J Transl Res; Over-expression of miR-193a-3p regulates the apoptosis of colorectal cancer cells by targeting PAK3.; Authors - Tao Ma
rat	WB	J Control Release; Dentin tubules as a long-term sustained release carrier to accelerate bone repair by loading FTY720; Authors - Jiaman Xie