ARL1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ARL1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 16012-1-AP

Synonyme

ADP ribosylation factor like 1, ADP-ribosylation factor-like protein 1

---

• 

  WB analysis using 16012-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16012-1-AP (ARL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16012-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16012-1-AP (ARL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16012-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16012-1-AP (ARL1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16012-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16012-1-AP (ARL1 antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16012-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16012-1-AP (ARL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Neuro-2a using 16012-1-AP

  IP result of anti-ARL1 (IP:16012-1-AP, 4ug; Detection:16012-1-AP 1:4000) with Neuro-2a cells lysate 2400 ug.

• 

  IHC staining of mouse brain using 16012-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16012-1-AP (ARL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 16012-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16012-1-AP (ARL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16012-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARL1 antibody (16012-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, C6-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, Mauslebergewebe, Neuro-2a-Zellen
• Erfolgreiche IP: Neuro-2a-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

16012-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ARL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARL1 fusion protein Ag8770
• Vollständiger Name: ADP-ribosylation factor-like 1
• Berechnetes Molekulargewicht: 181 aa, 20 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC007000
• Gene symbol: ARL1
• Gene ID (NCBI): 400
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell; The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins; Authors - Huaqing Deng
human	WB	Nature; FSP1 is a glutathione-independent ferroptosis suppressor.; Authors - Sebastian Doll
human	WB,IP	Nat Protoc; Proteome-wide analysis of protein lipidation using chemical probes: in-gel fluorescence visualization, identification and quantification of N-myristoylation, N- and S-acylation, O-cholesterylation, S-farnesylation and S-geranylgeranylation.; Authors - Wouter W Kallemeijn
human	WB	Nat Commun; Rab27a co-ordinates actin-dependent transport by controlling organelle-associated motors and track assembly proteins.; Authors - Noura Alzahofi
human	WB	J Cell Biol; ARFRP1 functions upstream of ARL1 and ARL5 to coordinate recruitment of distinct tethering factors to the trans-Golgi network.; Authors - Morié Ishida ; KO Validated
human	IF	Cell Rep; Role for the IFT-A Complex in Selective Transport to the Primary Cilium.; Authors - Wenxiang Fu