ARL2BP Polyklonaler Antikörper

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• KD/KO Validated

ARL2BP Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 10090-2-AP

Synonyme

RP66, BART1, BART, ARF-like 2-binding protein, ADP-ribosylation factor-like protein 2-binding protein

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  WB analysis of A549 using 10090-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10090-2-AP (ARL2BP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 10090-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10090-2-AP (ARL2BP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10090-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10090-2-AP (ARL2BP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Produktinformation

10090-2-AP bindet in WB, IHC, IF, IP, ELISA ARL2BP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARL2BP fusion protein Ag0138
• Vollständiger Name: ADP-ribosylation factor-like 2 binding protein
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC003087
• Gene symbol: ARL2BP
• Gene ID (NCBI): 23568
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARF-like proteins (ARLs) belong to a group of incompletely characterized members in the ARF family of RAS-related GTPases. A 19-kDa protein (BART, Binder of Arl Two) was identified to bind specifically to ARL2.GDP with high affinity but not with ARL2.GDP. In human, the 489-base pair BART open reading frame encodes a novel 163-amino acid protein with a predicted molecular mass of 18,822 Da. Data from Northern and Western analyses indicated BART is expressed in many tissues.

Publikationen

Species	Application	Title
mouse	WB,IF	Am J Hum Genet; Mutations in ARL2BP, Encoding ADP-Ribosylation-Factor-Like 2 Binding Protein, Cause Autosomal-Recessive Retinitis Pigmentosa.; Authors - Davidson Alice E AE ; KD Validated
human	WB	Cancer Res; Intracellular CD24 inhibits cell invasion by posttranscriptional regulation of BART through interaction with G3BP.; Authors - Keisuke Taniuchi
human	WB,IF	Neoplasia; BART inhibits pancreatic cancer cell invasion by Rac1 inactivation through direct binding to active Rac1.; Authors - Taniuchi Keisuke K ; KD Validated
human	WB,IF	PLoS One; BART inhibits pancreatic cancer cell invasion by PKCα inactivation through binding to ANX7.; Authors - Taniuchi Keisuke K ; KD Validated
human	WB,IF	Invest Ophthalmol Vis Sci; Truncation mutation in HRG4 (UNC119) leads to mitochondrial ANT-1-mediated photoreceptor synaptic and retinal degeneration by apoptosis.; Authors - Mori Naoki N
human	WB,IF,IP	Int J Oncol; BART inhibits pancreatic cancer cell invasion by inhibiting ARL2-mediated RhoA inactivation.; Authors - Taniuchi Keisuke K

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