ARMC10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ARMC10 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 20506-1-AP

Synonyme

armadillo repeat containing 10, Armadillo repeat-containing protein 10, PNAS 112, PSEC0198, SVH

---

• 

  WB analysis using 20506-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20506-1-AP (ARMC10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20506-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20506-1-AP (ARMC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20506-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20506-1-AP (ARMC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20506-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20506-1-AP (ARMC10 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20506-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20506-1-AP (ARMC10 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 20506-1-AP

  IP result of anti-ARMC10 (IP:20506-1-AP, 4ug; Detection:20506-1-AP 1:500) with A431 cells lysate 1600ug.

• 

  IHC staining of human placenta using 20506-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 20506-1-AP (ARMC10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 20506-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 20506-1-AP (ARMC10 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 20506-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ARMC10 antibody (20506-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

• 

  IF Staining of A431 using 20506-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ARMC10 antibody (20506-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

• 

  IF Staining of A431 using 20506-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 20506-1-AP (ARMC10 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HEK-293-Zellen, HepG2-Zellen, Maushirngewebe, Mauslebergewebe, Rattenherzgewebe
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

20506-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ARMC10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARMC10 fusion protein Ag14313
• Vollständiger Name: armadillo repeat containing 10
• Berechnetes Molekulargewicht: 343 aa, 38 kDa
• Beobachtetes Molekulargewicht: 31-37 kDa
• GenBank-Zugangsnummer: BC003586
• Gene symbol: ARMC10
• Gene ID (NCBI): 83787
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IP	Cell Death Dis; The Armc10/SVH gene: genome context, regulation of mitochondrial dynamics and protection against Aβ-induced mitochondrial fragmentation.; Authors - R Serrat
human	WB	Heliyon; Prognostic value and immune infiltration of ARMC10 in pancreatic adenocarcinoma via integrated bioinformatics analyses; Authors - Tian-Hao Li ; KD Validated
human	WB,IF	J Cell Mol Med; ARMC10 regulates mitochondrial dynamics and affects mitochondrial function via the Wnt/β-catenin signalling pathway involved in ischaemic stroke; Authors - Yanyang Huang ; KD Validated
human	IHC	BMC Cancer; Comprehensive analysis of the expression and prognostic value of ARMCs in pancreatic adenocarcinoma; Authors - Guanxiang Zhuo
human	WB,IHC,IF	Mol Carcinog; ARMC10 Drives Glioblastoma Progression Through Activating Notch Pathway; Authors - Bin Feng ; KD Validated