ARPC1B Polyklonaler Antikörper

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• KD/KO Validated

ARPC1B Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 28368-1-AP

Synonyme

Actin-related protein 2/3 complex subunit 1B, ARC41, p41 ARC, p41-ARC

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  WB analysis of HeLa using 28368-1-AP

  WB result of ARPC1B antibody (28368-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARPC1B transfected HeLa cells.

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  WB analysis using 28368-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28368-1-AP (ARPC1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 28368-1-AP

  IP result of anti-ARPC1B (IP:28368-1-AP, 4ug; Detection:28368-1-AP 1:1000) with HeLa cells lysate 1600 ug.

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  IHC staining of human colon cancer using 28368-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28368-1-AP (ARPC1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28368-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28368-1-AP (ARPC1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 28368-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28368-1-AP (ARPC1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 28368-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28368-1-AP (ARPC1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 28368-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28368-1-AP (ARPC1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, Maushirngewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

28368-1-AP bindet in WB, IHC, IP, ELISA ARPC1B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARPC1B fusion protein Ag29001
• Vollständiger Name: actin related protein 2/3 complex, subunit 1B, 41kDa
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC007555
• Gene symbol: ARPC1B
• Gene ID (NCBI): 10095
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARPC1B also known as ARC41, p41-ARC, or p40-ARC, is one of seven subunits of the human Arp2/3 protein complex, that belongs to the SOP2 family. ARPC1B localizes on centrosomes and has a distinct role in centrosomal homeostasis. ARPC1B is both a physiological activator and substrate of Aurora A kinase, and these interactions help to maintain mitotic integrity in mammalian cells. In recent resaeach, ARPC1B is selected as a candidate prediction marker for sensitivity of Choroidal malignant melanomas to radiotherapy.

Publikationen

Species	Application	Title
mouse,human	WB	Front Immunol; The overexpression of actin related protein 2/3 complex subunit 1B(ARPC1B) promotes the ovarian cancer progression via activation of the Wnt/β-catenin signaling pathway; Authors - Junning Huang
human	IHC	J Transl Med; A multi-dimensional approach to unravel the intricacies of lactylation related signature for prognostic and therapeutic insight in colorectal cancer; Authors - Huixia Huang
human	WB,IHC	Mol Cell Probes; High expression of ARPC1B promotes the proliferation and Apoptosis of clear cell renal cell carcinoma cells, leading to a poor prognosis; Authors - Hongbo Wang ; KD Validated
mouse	WB	CNS Neurosci Ther; Integrated Mendelian Randomization and Single-Cell Transcriptomics Analysis Identifies Critical Blood Biomarkers and Potential Mechanisms in Epilepsy; Authors - Jianwei Shi