CLMP Polyklonaler Antikörper

CLMP Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 16127-1-AP

Synonyme

ACAM, adipocyte adhesion molecule, ASAM, CLMP, CXADR-like membrane protein

---

• 

  WB analysis using 16127-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16127-1-AP (CLMP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 16127-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 16127-1-AP (CLMP antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brown adipose using 16127-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 16127-1-AP (CLMP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 16127-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 16127-1-AP (CLMP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, 3T3-L1-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: braunes Mausfettgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

16127-1-AP bindet in WB, IHC, ELISA CLMP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CLMP fusion protein Ag9111
• Vollständiger Name: adipocyte-specific adhesion molecule
• Berechnetes Molekulargewicht: 373 aa, 41 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC009371
• Gene symbol: CLMP
• Gene ID (NCBI): 79827
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CLMP (Coxsackie- and adenovirus receptor-like membrane protein), also known as ACAM (adipocyte adhesion molecule) or ASAM, is a member of the CTX (cortical thymocyte marker in Xenopus) family of proteins. CTX family proteins are type I transmembrane proteins within the Ig superfamily that localize to junctional complexes between endothelial and epithelial cells and may play a role in cell-cell adhesion. CLMP is predominantly expressed in epithelial cells within different tissues and in the white adipose tissue. It may be involved in the cell-cell adhesion and may play a role in adipocyte differentiation and development of obesity.

Publikationen

Species	Application	Title
human	WB	Cell Microbiol; Porphyromonas gingivalis induces penetration of lipopolysaccharide and peptidoglycan through the gingival epithelium via degradation of coxsackievirus and adenovirus receptor.; Authors - Hiroki Takeuchi