ASCC2 Polyklonaler Antikörper

ASCC2 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 11529-1-AP

Synonyme

ASC-1 complex subunit p100, ASC1P100, RQT3

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  WB analysis using 11529-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 11529-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11529-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11529-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 11529-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11529-1-AP

  IP result of anti-ASCC2 (IP:11529-1-AP, 4ug; Detection:11529-1-AP 1:1000) with HeLa cells lysate 2640ug.

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  IHC staining of human colon cancer using 11529-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11529-1-AP (ASCC2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11529-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASCC2 antibody (11529-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of HepG2 using 11529-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ASCC2 Polyclonal antibody (11529-1-AP)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, humanes Hirngewebe, humanes Kolongewebe, Maushirngewebe, Maus-Kolongewebe, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

11529-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA ASCC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASCC2 fusion protein Ag2100
• Vollständiger Name: activating signal cointegrator 1 complex subunit 2
• Berechnetes Molekulargewicht: 757 aa, 86 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC025368
• Gene symbol: ASCC2
• Gene ID (NCBI): 84164
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ASCC2, also known as ASC1p100, is a subunit of the activating signal cointegrator 1 complex. ASCC2 plays a role in DNA damage repair as a component of the ASCC complex, recruiting ASCC3 and ALKBH3 to sites of DNA damage by binding to polyubiquitinated proteins that have 'Lys-63'-linked polyubiquitin chains. Additionally, it is part of the ASC-1 complex that enhances NF-kappa-B, SRF, and AP1 transactivation(PMID: 29144457, 12077347).

Publikationen

Species	Application	Title
human	WB	Nat Commun; Extended DNA threading through a dual-engine motor module of the activating signal co-integrator 1 complex; Authors - Junqiao Jia
	WB	Nat Commun; The interaction of DNA repair factors ASCC2 and ASCC3 is affected by somatic cancer mutations.; Authors - Junqiao Jia
mouse	WB	Chin Med; Du Huo Ji Sheng Tang inhibits Notch1 signaling and subsequent NLRP3 activation to alleviate cartilage degradation in KOA mice; Authors - Wen-Jin Chen