ASCC2 Polyklonaler Antikörper

ASCC2 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 11529-1-AP

Synonyme

ASC 1 complex subunit p100, ASC1p100, ASCC2, Trip4 complex subunit p100

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Galerie der Validierungsdaten

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  WB analysis using 11529-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11529-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 11529-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 11529-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11529-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11529-1-AP (ASCC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11529-1-AP

  IP Result of anti-ASCC2 (IP:11529-1-AP, 4ug; Detection:11529-1-AP 1:1000) with HeLa cells lysate 2640ug.

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  IHC staining of human colon cancer using 11529-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11529-1-AP (ASCC2 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 11529-1-AP

  Immunofluorescent analysis of HepG2 cells, using ASCC2 antibody 11529-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 11529-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug ASCC2 antibody (11529-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, humanes Hirngewebe, humanes Kolongewebe, Maushirngewebe, Maus-Kolongewebe, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

11529-1-AP bindet in WB, IP, IHC, IF, FC, ELISA ASCC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASCC2 fusion protein Ag2100
• Vollständiger Name: activating signal cointegrator 1 complex subunit 2
• Berechnetes Molekulargewicht: 757 aa, 86 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC025368
• Gene symbol: ASCC2
• Gene ID (NCBI): 84164
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Extended DNA threading through a dual-engine motor module of the activating signal co-integrator 1 complex; Authors - Junqiao Jia
	WB	Nat Commun; The interaction of DNA repair factors ASCC2 and ASCC3 is affected by somatic cancer mutations.; Authors - Junqiao Jia
mouse	WB	Chin Med; Du Huo Ji Sheng Tang inhibits Notch1 signaling and subsequent NLRP3 activation to alleviate cartilage degradation in KOA mice; Authors - Wen-Jin Chen