ASCC3 Polyklonaler Antikörper

ASCC3 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 17627-1-AP

Synonyme

Helicase, ATP binding 1, HELIC1, EC:3.6.4.12, ASC1p200, ASC-1 complex subunit p200



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, HepG2-Zellen
Erfolgreiche IPHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2400
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 7 publications below
IPSee 2 publications below

Produktinformation

17627-1-AP bindet in WB, IP, ELISA ASCC3 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ASCC3 fusion protein Ag11848
Vollständiger Name activating signal cointegrator 1 complex subunit 3
Berechnetes Molekulargewicht 111 aa, 13 kDa, 251 kDa
Beobachtetes Molekulargewicht 251 kDa
GenBank-ZugangsnummerBC050681
Gene symbol ASCC3
Gene ID (NCBI) 10973
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ASCC3 (Activating Signal Cointegrator 1 Complex Subunit 3) is a multifunctional protein involved in various cellular processes, including transcriptional regulation, DNA repair, and ribosome quality control. It is a member of the ASCC (Activating Signal Cointegrator 1 Complex) family, which functions as a bridge between co-repressors and co-activators in transcriptional regulation.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for ASCC3 antibody 17627-1-APProtokoll herunterladen
IP protocol for ASCC3 antibody 17627-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIP,WB

Nat Commun

Extended DNA threading through a dual-engine motor module of the activating signal co-integrator 1 complex

Authors - Junqiao Jia
humanWB

Nat Commun

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis

Authors - Ji Geng
humanWB

Mol Cell

Unresolved stalled ribosome complexes restrict cell-cycle progression after genotoxic stress.

Authors - Mark Stoneley
humanWB

Sci Rep

Identification of a novel trigger complex that facilitates ribosome-associated quality control in mammalian cells.

Authors - Satoshi Hashimoto
mouseWB

Cell Metab

Ribosome stalling is a signal for metabolic regulation by the ribotoxic stress response

Authors - Goda Snieckute
humanIP

EMBO J

The ASC-1 complex promotes translation initiation by scanning ribosomes

Authors - Yuki Kito

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Tom (Verified Customer) (10-24-2023)

(Already in contact with technical support, but was asked to submit a review here as well.) Immunoblotting for ASCC3 from fresh cell extracts did not work. Included samples of ASCC3-KD (siRNA) experiment, but only unspecific bands. Tested 1:1000 and 1:500 dilution. Tested different protein extraction buffers and conditions, used fresh cells each time. Transfer of large proteins was confirmed. Sadly, for us this antibody did not work.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000 and 1:500
  • Cell Tissue Type: HEK293