ASGR1 Monoklonaler Antikörper

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• KD/KO Validated

ASGR1 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, ELISA, Blocking assay

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1G5F5

Kat-Nr. : 66692-1-Ig

Synonyme

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

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  WB analysis of HepG2 using 66692-1-Ig

  WB result of ASGR1 antibody (66692-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ASGR1 transfected HepG2 cells.

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  WB analysis using 66692-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 66692-1-Ig

  WB result of ASGR1 antibody (66692-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ASGR1 transfected L02 cells.

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  WB analysis of mouse hepatocytes using 66692-1-Ig

  mouse hepatocytes were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 66692-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66692-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 66692-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66692-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66692-1-Ig (ASGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 66692-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66692-1-Ig (ASGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HuH-7-Zellen, HepG2-Zellen, L02-Zellen, Hausschwein-Lebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66692-1-Ig bindet in WB, IHC, ELISA, Blocking assay ASGR1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ASGR1 fusion protein Ag2310
• Vollständiger Name: asialoglycoprotein receptor 1
• Berechnetes Molekulargewicht: 291 aa, 33 kDa
• Beobachtetes Molekulargewicht: 42-46 kDa
• GenBank-Zugangsnummer: BC032130
• Gene symbol: ASGR1
• Gene ID (NCBI): 432
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

Publikationen

Species	Application	Title
human	WB,Blocking assay	J Med Virol; The use of human iPSC-derived alveolar organoids to explore SARS-CoV-2 variant infections and host responses; Authors - Chaitanya Gandikota