ASGR1 Polyklonaler Antikörper

ASGR1 Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen

1

Kat-Nr. : CL488-11739

Synonyme

ASGP R 1, ASGPR, ASGPR 1, ASGR1, asialoglycoprotein receptor 1, CLEC4H1, Hepatic lectin H1, HL 1, Hs.12056

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Galerie der Validierungsdaten

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  IF Staining of human liver cancer using CL488-11739

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 ASGR1 antibody (CL488-11739) at dilution of 1:200.

• 

  IF Staining of rat liver using CL488-11739

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using CoraLite® Plus 488 ASGR1 antibody (CL488-11739) at dilution of 1:200.

• 

  FC experiment of HepG2 using CL488-11739

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human ASGR1 (CL488-11739) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were not fixed .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Rattenlebergewebe, humanes Leberkarzinomgewebe
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL488-11739 bindet in IF, FC (Intra) ASGR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASGR1 fusion protein Ag2310
• Vollständiger Name: asialoglycoprotein receptor 1
• Berechnetes Molekulargewicht: 291 aa, 33 kDa
• GenBank-Zugangsnummer: BC032130
• Gene symbol: ASGR1
• Gene ID (NCBI): 432
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Publikationen

Species	Application	Title
mouse	IF	MedComm (2020); LILAR, a novel long noncoding RNA regulating autophagy in the liver tissues of endotoxemic mice through a competing endogenous RNA mechanism; Authors - Tian Tian