ASGR1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ASGR1 Polyklonal Antikörper für IF-P, FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF-P, FC

Konjugation

CoraLite®555 Fluorescent Dye

Rezensionen (2)

Kat-Nr. : CL555-11739

Synonyme

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

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  IF Staining of rat liver using CL555-11739

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using CoraLite®555 ASGR1 antibody (CL555-11739) at dilution of 1:200.

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  IF Staining of rat liver using CL555-11739

  Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using CoraLite®555 ASGR1 antibody (CL555-11739) at dilution of 1:200.

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  IF Staining of human liver cancer using CL555-11739

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®555 ASGR1 antibody (CL555-11739) at dilution of 1:200.

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  IF Staining of human liver cancer using CL555-11739

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®555 ASGR1 antibody (CL555-11739) at dilution of 1:200.

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  FC experiment of HepG2 using CL555-11739

  1X10^6 HepG2 cells were surface stained with 0.4 ug CoraLite®555 Anti-Human ASGR1 (CL555-11739) (red), or 0.4 ug Control Antibody. Cells were not fixed .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Rattenlebergewebe, humanes Leberkarzinomgewebe
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-11739 bindet in IF-P, FC ASGR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASGR1 fusion protein Ag2310
• Vollständiger Name: asialoglycoprotein receptor 1
• Berechnetes Molekulargewicht: 291 aa, 33 kDa
• GenBank-Zugangsnummer: BC032130
• Gene symbol: ASGR1
• Gene ID (NCBI): 432
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rupa (Verified Customer) (04-26-2024)

Worked very well with immunofluorescence and flowcytometry

• Applications: Immunohistochemistry, Flow Cytometry
• Primary Antibody Dilution: 1-100
• Cell Tissue Type: mouse liver

FH

Parmita (Verified Customer) (04-23-2024)

worked fine for us. signal was sharp and specific detection observed

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse liver tissue