ASK1 Monoklonaler Antikörper

ASK1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

CloneNo.

1C3A10

Kat-Nr. : 67072-1-Ig

Synonyme

ASK 1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5

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Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 67072-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67072-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67072-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67072-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67072-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67072-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 67072-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 67072-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 67072-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 67072-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67072-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67072-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of RAW 264.7 using 67072-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using ASK1 antibody (67072-1-Ig, Clone: 1C3A10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HSC-T6 cells, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Lungenkarzinomgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:150-1:600
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 2 publications below

Produktinformation

67072-1-Ig bindet in WB, IHC, IF, ELISA ASK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ASK1 fusion protein Ag5818
• Vollständiger Name: mitogen-activated protein kinase kinase kinase 5
• Berechnetes Molekulargewicht: 155 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC054503
• Gene symbol: MAP3K5
• Gene ID (NCBI): 4217
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. 67072-1-Ig antibody detects the degraded product of ASK1 in SDS-PAGE.

Publikationen

Species	Application	Title
human,rat	WB	Aging (Albany NY); Jian-Pi-Yi-Shen decoction inhibits mitochondria-dependent granulosa cell apoptosis in a rat model of POF; Authors - Xiao-Lin Jiang
rat	WB	Front Endocrinol (Lausanne); Cangfudaotan decoction inhibits mitochondria-dependent apoptosis of granulosa cells in rats with polycystic ovarian syndrome; Authors - Xiao-Lin Jiang
human	WB	Cells; Orphan Nuclear Receptor Nur77 Mediates the Lethal Endoplasmic Reticulum Stress and Therapeutic Efficacy of Cryptomeridiol in Hepatocellular Carcinoma; Authors - Xudan Li
rat	IF	Mol Med Rep; ASK1 inhibitor NQDI‑1 decreases oxidative stress and neuroapoptosis via the ASK1/p38 and JNK signaling pathway in early brain injury after subarachnoid hemorrhage in rats; Authors - Jiajia Duan
rat	WB,IF	Phytomedicine; Exploring the mechanism of Xingpi Capsule in diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology; Authors - Weina Qian
mouse		Front Pharmacol; Erratum: Addendum: A novel N-Arylpyridone compound alleviates the inflammatory and fibrotic reaction of silicosis by inhibiting the ASK1-p38 pathway and regulating macrophage polarization; Authors - Mingming Fan