ASPM Polyklonaler Antikörper

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• KD/KO Validated

ASPM Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Kat-Nr. : 26223-1-AP

Synonyme

ASP, Asp homolog, ASPM, Calmbp1, MCPH5

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  WB analysis using 26223-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26223-1-AP (ASPM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 26223-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 26223-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26223-1-AP (ASPM Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary tumor using 26223-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26223-1-AP (ASPM Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human gliomas using 26223-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 26223-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 26223-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 26223-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 26223-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26223-1-AP (ASPM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 26223-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 26223-1-AP (ASPM antibody), at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with alpha tubulin (66031-1-Ig, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Gliomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 9 publications below
• IHC: See 9 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

26223-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA ASPM und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASPM fusion protein Ag24166
• Vollständiger Name: asp (abnormal spindle) homolog, microcephaly associated (Drosophila)
• Berechnetes Molekulargewicht: 410 kDa or 218 kDa
• Beobachtetes Molekulargewicht: 218 kDa
• GenBank-Zugangsnummer: BC034607
• Gene symbol: ASPM
• Gene ID (NCBI): 259266
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB,IHC,IP	Aging (Albany NY); ASPM promotes glioblastoma growth by regulating G1 restriction point progression and Wnt-β-catenin signaling.; Authors - Xin Chen ; KD Validated
human	IHC	Int J Cancer; Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.; Authors - Fangqiu Fu
human	WB	Front Oncol; Identifying and Validating Potential Biomarkers of Early Stage Lung Adenocarcinoma Diagnosis and Prognosis.; Authors - Yingji Chen
mouse	IHC	J Biol Chem; The Transcription factor NF-YA is Crucial for Neural Progenitor Maintenance during Brain Development; Authors - Tomoyuki Yamanaka
human	WB	DNA Cell Biol; ASPM is a Novel Candidate Gene Associated with Colorectal Cancer Cell Growth.; Authors - Qian Yang
human	WB,IHC	Int J Endocrinol; ASPM Promotes the Progression of Anaplastic Thyroid Carcinomas by Regulating the Wnt/β-Catenin Signaling Pathway.; Authors - Liang Jiang ; KD Validated