ASS1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ASS1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(36)

Kat-Nr. : 16210-1-AP

Synonyme

EC:6.3.4.5, ASS

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  WB analysis using 16210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 16210-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 16210-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16210-1-AP

  IP result of anti-ASS1 (IP:16210-1-AP, 4ug; Detection:16210-1-AP 1:1500) with HeLa cells lysate 1600ug.

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  IHC staining of human testis using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16210-1-AP (ASS1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16210-1-AP (ASS1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human placenta using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16210-1-AP (ASS1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16210-1-AP (ASS1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16210-1-AP (ASS1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16210-1-AP (ASS1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16210-1-AP (ASS1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16210-1-AP (ASS1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16210-1-AP (ASS1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16210-1-AP (ASS1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 16210-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ASS1 antibody (16210-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, Mausnierengewebe, Rattennierengewebe, SMMC-7721-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Leberkarzinomgewebe, humanes Lebergewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 28 publications below
• IHC: See 14 publications below
• IF: See 6 publications below
• CoIP: See 2 publications below

Produktinformation

16210-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA ASS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASS1 fusion protein Ag9200
• Vollständiger Name: argininosuccinate synthetase 1
• Berechnetes Molekulargewicht: 412 aa, 47 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC009243
• Gene symbol: ASS1
• Gene ID (NCBI): 445
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).

Publikationen

Species	Application	Title
mouse	IF	Immunity; Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.; Authors - Fangzhou Lou
human	WB	Sci Transl Med; An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs.; Authors - Wei-Jian Li
human	WB	Mol Cell; Arginine deprivation enriches lung cancer proteomes with cysteine by inducing arginine-to-cysteine substitutants; Authors - Chao Yang
human	WB	Mol Cell; CLOCK Acetylates ASS1 to Drive Circadian Rhythm of Ureagenesis.; Authors - Ran Lin ; KO Validated
mouse	IHC	Autophagy; BNIP3-dependent mitophagy promotes cytosolic localization of LC3B and metabolic homeostasis in the liver.; Authors - Maya Z Springer
mouse	IF	J Am Soc Nephrol; Hnf4a Is Required for the Development of Cdh6-Expressing Progenitors into Proximal Tubules in the Mouse Kidney.; Authors - Sierra S Marable