ASS1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

ASS1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

1D1A11

Kat-Nr. : 66036-1-Ig

Synonyme

Argininosuccinate synthase, argininosuccinate synthetase 1, ASS, ASS1, Citrulline aspartate ligase, CTLN1

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Galerie der Validierungsdaten

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  WB analysis of pig liver using 66036-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66036-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66036-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 66036-1-Ig

  human liver tissue were subjected to SDS PAGE followed by western blot with 66036-1-Ig (ASS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66036-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human liver using 66036-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 40x lens).

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  IHC staining of human testis using 66036-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 66036-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 66036-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66036-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66036-1-Ig (ASS1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66036-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 66036-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver using 66036-1-Ig(ASS1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 66036-1-Ig

  Immunofluorescent analysis of MCF-7 cells, using ASS1 antibody 66036-1-Ig at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Lebergewebe, HeLa-Zellen, humanes Lebergewebe, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Hodengewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

66036-1-Ig bindet in WB, IHC, IF ASS1 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ASS1 fusion protein Ag9314
• Vollständiger Name: argininosuccinate synthetase 1
• Berechnetes Molekulargewicht: 412 aa, 47 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC009243
• Gene symbol: ASS1
• Gene ID (NCBI): 445
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).

Publikationen

Species	Application	Title
human	WB	FASEB J; Argininosuccinate synthetase 1 contributes to gastric cancer invasion and progression by modulating autophagy.; Authors - Chung-Ying Tsai ; KD Validated
human	WB	Mol Pharmacol; MicroRNA-1291-5p Sensitizes Pancreatic Carcinoma Cells to Arginine Deprivation and Chemotherapy through the Regulation of Arginolysis and Glycolysis.; Authors - Mei-Juan Tu
human	WB	BMC Cancer; PMID: 25240403; Authors - Jiangbo Liu
human	WB	BMC Cancer; Intracellular expression of arginine deiminase activates the mitochondrial apoptosis pathway by inhibiting cytosolic ferritin and inducing chromatin autophagy.; Authors - Qingyuan Feng