ATF4 Polyklonaler Antikörper

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• KD/KO Validated

ATF4 Polyklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, FC (Intra)

Konjugation

FITC Fluorescent Dye

Publikationen(5)

Kat-Nr. : FITC-10835

Synonyme

cAMP-dependent transcription factor ATF-4, CREB2, Cyclic AMP-responsive element-binding protein 2, Tax-responsive enhancer element-binding protein 67, TXREB

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  IF Staining of HeLa using FITC-10835

  Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using FITC ATF4 antibody (FITC-10835) at dilution of 1:200, CL594-Phalloidin (red).

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  IF Staining of HeLa using FITC-10835

  Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using FITC ATF4 antibody (FITC-10835) at dilution of 1:200, CL594-phalloidin (red).

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  FC experiment of HeLa using FITC-10835

  1X10^6 HeLa cells were intracellularly stained with 0.6 ug FITC Anti-Human ATF4 (FITC-10835) (red), or 0.6 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: mit Tunicamycin behandelte HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.60 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 3 publications below

Produktinformation

FITC-10835 bindet in WB, IF/ICC, FC (Intra) ATF4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATF4 fusion protein Ag1279
• Vollständiger Name: activating transcription factor 4 (tax-responsive enhancer element B67)
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC022088
• Gene symbol: ATF4
• Gene ID (NCBI): 468
• Konjugation: FITC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 498 nm / 526 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation[PMID: 19016586]. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies[PMID: 19726872]. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia[PMID: 18163433]. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer. Endogenous ATF4 protein has a molecular mass of 50kd. [PMID: 17726049]. This antibody is a rabbit polyclonal antibody raised against full length human ATF4 antigen. The antibody recognizes the 38kd ATF4 protein and its phosphorylated forms (50kd). ATF4 can bind DNA as a homodimer and as a heterodimer. ATF4 is ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4, so the molecular weight of ATF4 may be 70 kDa.

Publikationen

Species	Application	Title
Mouse	IF	Oxid Med Cell Longev; Protocatechuic Acid-Mediated miR-219a-5p Activation Inhibits the p66shc Oxidant Pathway to Alleviate Alcoholic Liver Injury.; Authors - Rong Fu
human	IF	Cancer Cell Int; MiR-876-5p modulates head and neck squamous cell carcinoma metastasis and invasion by targeting vimentin.; Authors - Yibo Dong
	IF	Mol Med Rep; Molecular mechanism underlying miR‑130b‑Sp1 transcriptional regulation in LPS‑induced upregulation of MUC5AC in the bile duct epithelium.; Authors - Xiaodong Wu
Human	WB	Front Pharmacol; Polydatin enhances oxaliplatin-induced cell death by activating NOX5-ROS-mediated DNA damage and ER stress in colon cancer cells; Authors - Qi Zhao
		Int J Nanomedicine; Nanoparticle/Engineered Bacteria Based Triple-Strategy Delivery System for Enhanced Hepatocellular Carcinoma Cancer Therapy; Authors - Meiyang Yang