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- KD/KO Validated
ATF4 Polyklonaler Antikörper
ATF4 Polyklonal Antikörper für IF/ICC, FC (Intra)
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus
Anwendung
WB, IF/ICC, FC (Intra)
Konjugation
FITC Fluorescent Dye
Kat-Nr. : FITC-10835
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in IF/ICC | mit Tunicamycin behandelte HeLa-Zellen | 
| Erfolgreiche Detektion in FC (Intra) | HeLa-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 | 
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.60 ug per 10^6 cells in a 100 µl suspension | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 1 publications below | 
| IF | See 3 publications below | 
Produktinformation
FITC-10835 bindet in WB, IF/ICC, FC (Intra) ATF4 und zeigt Reaktivität mit human, Maus
| Getestete Reaktivität | human, Maus | 
| In Publikationen genannte Reaktivität | human, Maus | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Polyklonal | 
| Typ | Antikörper | 
| Immunogen | ATF4 fusion protein Ag1279 | 
| Vollständiger Name | activating transcription factor 4 (tax-responsive enhancer element B67) | 
| Berechnetes Molekulargewicht | 39 kDa | 
| Beobachtetes Molekulargewicht | 45-50 kDa | 
| GenBank-Zugangsnummer | BC022088 | 
| Gene symbol | ATF4 | 
| Gene ID (NCBI) | 468 | 
| Konjugation | FITC Fluorescent Dye | 
| Excitation/Emission maxima wavelengths | 498 nm / 526 nm | 
| Form | Liquid | 
| Reinigungsmethode | Antigen-Affinitätsreinigung | 
| Lagerungspuffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA | 
| Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation[PMID: 19016586]. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies[PMID: 19726872]. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia[PMID: 18163433]. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer. Endogenous ATF4 protein has a molecular mass of 50kd. [PMID: 17726049]. This antibody is a rabbit polyclonal antibody raised against full length human ATF4 antigen. The antibody recognizes the 38kd ATF4 protein and its phosphorylated forms (50kd). ATF4 can bind DNA as a homodimer and as a heterodimer. ATF4 is ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4, so the molecular weight of ATF4 may be 70 kDa.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| IF protocol for FITC ATF4 antibody FITC-10835 | Protokoll herunterladen | 
| FC protocol for FITC ATF4 antibody FITC-10835 | Download protocol | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Oxid Med Cell Longev Protocatechuic Acid-Mediated miR-219a-5p Activation Inhibits the p66shc Oxidant Pathway to Alleviate Alcoholic Liver Injury. | ||
| Cancer Cell Int MiR-876-5p modulates head and neck squamous cell carcinoma metastasis and invasion by targeting vimentin. | ||
| Mol Med Rep Molecular mechanism underlying miR‑130b‑Sp1 transcriptional regulation in LPS‑induced upregulation of MUC5AC in the bile duct epithelium. | ||
| Front Pharmacol Polydatin enhances oxaliplatin-induced cell death by activating NOX5-ROS-mediated DNA damage and ER stress in colon cancer cells | ||
| Int J Nanomedicine Nanoparticle/Engineered Bacteria Based Triple-Strategy Delivery System for Enhanced Hepatocellular Carcinoma Cancer Therapy | 


