ATG16L2 Polyklonaler Antikörper

ATG16L2 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 24322-1-AP

Synonyme

APG16 like 2, ATG16L2, Autophagy related protein 16 2, WDR80

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  WB analysis of mouse spleen using 24322-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 24322-1-AP (ATG16L2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 24322-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24322-1-AP (ATG16L2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 24322-1-AP

  IP result of anti-ATG16L2 (IP:24322-1-AP, 3ug; Detection:24322-1-AP 1:700) with mouse testis tissue lysate 4800ug.

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  IF Staining of MCF-7 using 24322-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 24322-1-AP (ATG16L2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

24322-1-AP bindet in WB, IF/ICC, IP, ELISA ATG16L2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATG16L2 fusion protein Ag19399
• Vollständiger Name: ATG16 autophagy related 16-like 2 (S. cerevisiae)
• Berechnetes Molekulargewicht: 619 aa, 69 kDa
• Beobachtetes Molekulargewicht: 70 kDa, 30 kDa
• GenBank-Zugangsnummer: BC137490
• Gene symbol: ATG16L2
• Gene ID (NCBI): 89849
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATG16L2 also named as Autophagy related protein 16-2 is a 619 amino-acid protein, Which belongs to the WD repeat ATG16 family. ATG16L2 contains 7 WD repeats and localizes in the cytoplasm. There are three isoforms of ATG16L2 and the molecular weight of three isoform is respectively 69kDa, 56kDa and 31kDa. ATG16L2 may play a role in autophagy.

Publikationen

Species	Application	Title
mouse	WB	Gene; Intron retention is a stress response in sensor genes and is restored by Japanese herbal medicines: A basis for future clinical applications.; Authors - Trieu-Duc Vu