ATG2A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ATG2A Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA, Peptide array

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 23226-1-AP

Synonyme

KIAA0404, Autophagy-related protein 2 homolog A

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  WB analysis using 23226-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23226-1-AP (ATG2A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 23226-1-AP

  IP result of anti-ATG2A (IP:23226-1-AP, 4ug; Detection:23226-1-AP 1:4000) with K-562 cells lysate 2400 ug.

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  IHC staining of mouse kidney using 23226-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23226-1-AP (ATG2A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 23226-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23226-1-AP (ATG2A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 23226-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ATG2A antibody (23226-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, MCF-7-Zellen, Raji-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

23226-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA, Peptide array ATG2A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATG2A fusion protein Ag19709
• Vollständiger Name: ATG2 autophagy related 2 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 1938 aa, 213 kDa
• Beobachtetes Molekulargewicht: 215 kDa
• GenBank-Zugangsnummer: BC110650
• Gene symbol: ATG2A
• Gene ID (NCBI): 23130
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Autophagy-related protein 2 homolog A (ATG2A) belongs to the ATG2 family. ATG2A is a lipid transfer protein involved in autophagosome assembly (PMID:31271352). ATG2A tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion. ATG2A binds to the ER exit site (ERES), which is the membrane source for autophagosome formation, and extracts phospholipids from the membrane source and transfers them to ATG9 (ATG9A or ATG9B) to the IM for membrane expansion (PMID: 30952800, PMID: 31271352). ATG2A also regulates lipid droplet morphology and distribution within the cell (PMID: 28561066). The observed MW of ATG2A is 215 kDa.

Publikationen

Species	Application	Title
human	WB	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan
human	WB	Nat Cell Biol; ATG9A protects the plasma membrane from programmed and incidental permeabilization.; Authors - Aurore Claude-Taupin ; KO Validated
human	WB,CoIP,Peptide array	Mol Cell; ATG9A and ATG2A form a heteromeric complex essential for autophagosome formation; Authors - Alexander R van Vliet
human	IF	Nat Commun; The autophagy protein ATG9A enables lipid mobilization from lipid droplets.; Authors - Elodie Mailler ; KO Validated
human	WB,IF	EMBO Rep; A conserved ATG2-GABARAP family interaction is critical for phagophore formation.; Authors - Mihaela Bozic
human	WB	Theranostics; The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer.; Authors - Wu Sun