ATG3 Polyklonaler Antikörper

ATG3 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 11262-2-AP

Synonyme

APG3, APG3 LIKE, APG3L, ATG3, Autophagy related protein 3, hApg3, PC3 96, Protein PC3 96

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 11262-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11262-2-AP (ATG3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 11262-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11262-2-AP (ATG3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11262-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11262-2-AP (ATG3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 11262-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 11262-2-AP (ATG3 Antibody) at dilution of 1:50.

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  IHC staining of human kidney using 11262-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 11262-2-AP (ATG3 Antibody) at dilution of 1:50.

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  IF Staining of HEK-293 using 11262-2-AP

  Immunofluorescent analysis of HEK-293 cells using 11262-2-AP (ATG3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Mausnierengewebe, PC-12-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11262-2-AP bindet in WB, IHC, IF, ELISA ATG3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATG3 fusion protein Ag1785
• Vollständiger Name: ATG3 autophagy related 3 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36-40 kDa
• GenBank-Zugangsnummer: BC024221
• Gene symbol: ATG3
• Gene ID (NCBI): 64422
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; Cytoplasmic fragment of CD147 generated by regulated intramembrane proteolysis contributes to HCC by promoting autophagy.; Authors - Bo Wu
pig	WB	Chemosphere; Genome-wide transcriptional profiling and functional analysis reveal miR-330-MAPK15 axis involving in cellular responses to deoxynivalenol exposure.; Authors - Haifei Wang
mouse	WB	Front Cell Dev Biol; Aquaporin-8 transports hydrogen peroxide to regulate granulosa cell autophagy; Authors - Binbin Huang
mouse	WB	Front Immunol; Toxoplasma gondii CDPK3 Controls the Intracellular Proliferation of Parasites in Macrophages.; Authors - Minmin Wu
human	WB	Oncotarget; Upregulation of the lncRNA Meg3 induces autophagy to inhibit tumorigenesis and progression of epithelial ovarian carcinoma by regulating activity of ATG3.; Authors - Yin-Ling Xiu
mouse	WB	Ecotoxicol Environ Saf; Exposure to DEHP induces testis toxicity and injury through the ROS/mTOR/NLRP3 signaling pathway in immature rats.; Authors - Yifan Hong