ATOH1 Polyklonaler Antikörper

ATOH1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), chIP, ELISA

Konjugation

Unkonjugiert

Publikationen(50)

Rezensionen (7)

Kat-Nr. : 21215-1-AP

Synonyme

ATH1, bHLHa14, Class A basic helix-loop-helix protein 14, HATH1, Helix-loop-helix protein hATH-1

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  WB analysis using 21215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21215-1-AP (ATOH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21215-1-AP (ATOH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21215-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21215-1-AP (ATOH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21215-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21215-1-AP (ATOH1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 21215-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 21215-1-AP (ATOH1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 21215-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 21215-1-AP (ATOH1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 21215-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATOH1 antibody (21215-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 21215-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATOH1 antibody (21215-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

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  IF Staining of HepG2 using 21215-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 21215-1-AP (ATOH1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 21215-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human ATOH1 (21215-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, C6-Zellen, HepG2-Zellen, Maushirngewebe, Maus-Dünndarmgewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 23 publications below
• IHC: See 4 publications below
• IF: See 22 publications below
• ChIP: See 1 publications below

Produktinformation

21215-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), chIP, ELISA ATOH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATOH1 fusion protein Ag15615
• Vollständiger Name: atonal homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 354 aa, 38 kDa
• Beobachtetes Molekulargewicht: 38-40 kDa
• GenBank-Zugangsnummer: BC069594
• Gene symbol: ATOH1
• Gene ID (NCBI): 474
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATOH1, also named as Protein atonal homolog 1 or BHLHA14, is a 364 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain. ATOH1,as a transcriptional regulator in the nucleus activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. ATOH1 plays a role in the differentiation of subsets of neural cells by activating E box-dependent transcription.

Publikationen

Species	Application	Title
human	IF	Cell Res; Decoding the spatiotemporal regulation of transcription factors during human spinal cord development; Authors - Yingchao Shi
human	IF	Immunity; IL-17RA-signaling in Lgr5+ intestinal stem cells induces expression of transcription factor ATOH1 to promote secretory cell lineage commitment.; Authors - Xun Lin
human	WB	Nat Cell Biol; Merkel cell polyomavirus activates LSD1-mediated blockade of non-canonical BAF to regulate transformation and tumorigenesis.; Authors - Donglim Esther Park
mouse	IHC	J Clin Invest; Sox2 haploinsufficiency primes regeneration and Wnt responsiveness in the mouse cochlea.; Authors - Patrick J Atkinson
	IF	Dev Cell; Self-organized pattern formation in the developing mouse neural tube by a temporal relay of BMP signaling; Authors - Stefanie Lehr
mouse	IF	Proc Natl Acad Sci U S A; Sufu- and Spop-mediated regulation of Gli2 is essential for the control of mammalian cochlear hair cell differentiation; Authors - Tianli Qin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nicole (Verified Customer) (08-08-2024)

Works really well both in Western Blot and Immunofluorescence

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:500

FH

Yin (Verified Customer) (12-20-2021)

It did not work.

• Applications: Immunofluorescence
• Cell Tissue Type: Cerebella granule cell

FH

Stephen (Verified Customer) (11-04-2020)

ATOH1 antibody was tested on embryonic chicken tissue. Staining showed nuclear localisation in inner ear vestibular crista in sox2 positive prosensory region of 6 day old embryo

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Embryonic chicken inner ear tissue

ATOH1 Antibody Immunofluorescence validation (1:300 dilution) in Embryonic chicken inner ear tissue (Cat no:21215-1-AP)

FH

Roland (Verified Customer) (09-17-2020)

We tested several ATOH1 antibodies and this is the only one which turned out to be specific as confirmed bei knockdown experiments.

• Applications: Western Blot
• Cell Tissue Type: human cancer cells

FH

Biren (Verified Customer) (08-31-2020)

I used human GBM stem cells as a negative control to validate the specificity of this antibody. The cells used were confirmed by RNAseq to not express ATOH1, yet all cells were positively stained at the previously used concentration of 1/500, indicating that this antibody is not specific.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: human glioblastoma stem cells

ATOH1 Antibody Immunofluorescence validation (1/500 dilution) in human glioblastoma stem cells (Cat no:21215-1-AP)

FH

Erithelgi (Verified Customer) (09-25-2019)

The antibody detects a band of the expected size, in cells that induce GFP-ATOH1 under a DOX promoter. No bands detected in non-induced cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: medulloblastoma

FH

Nicole (Verified Customer) (07-23-2019)

Used to stain cultured stem cells for differentiation markers.

• Applications: Immunohistochemistry, Immunofluorescence,
• Cell Tissue Type: Stem Cell culture