ATP1A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ATP1A1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(257)

Rezensionen (3)

Kat-Nr. : 14418-1-AP

Synonyme

ATP1A 1, Atpa 1, EC:7.2.2.13, Na(+)/K(+) ATPase 1, Na(+)/K(+) ATPase alpha-1 subunit

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• Validation Data Gallery
• Proteintech vs. Other Vendors

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  WB analysis using 14418-1-AP

  Boiled and 37°C incubated mouse brain tissue were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14418-1-AP

  Boiled and 37°C incubated HEK-293 cells were subjected to SDS PAGE followed by western blot with 14418-1-AP (ATP1A1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14418-1-AP

  WB result of ATP1A1 antibody (14418-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP1A1 transfected HEK-293 cells.

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  IP experiment of HEK-293 using 14418-1-AP

  IP result of anti-ATP1A1 (IP:14418-1-AP, 4ug; Detection:14418-1-AP 1:20000) with HEK-293 cells lysate 1470 ug.

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  IHC staining of human liver cancer using 14418-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary tumor using 14418-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP (ATP1A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 14418-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14418-1-AP (ATP1A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 14418-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP (ATP1A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14418-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 14418-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 14418-1-AP (ATP1A1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 14418-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 14418-1-AP (ATP1A1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 14418-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human ovary tumor using 14418-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14418-1-AP (ATP1A1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of Caco-2 using 14418-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed Caco-2 cells using ATP1A1 antibody (14418-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 14418-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using ATP1A1 antibody (14418-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293 using 14418-1-AP

  1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug ATP1A1 Polyclonal antibody (14418-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  CoIP experiment of A431 using 14418-1-AP

  Co-IP result of anti-ATP1A1 (IP:14418-1-AP, 4ug; Detection:15192-1-AP 1:5000) with A431 cells lysate 1000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: bei 37°C inkubiertes Maushirngewebe, 37°C incubated HEK-293 cells
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Zervixkarzinomgewebe, humanes Kolongewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Caco-2-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen
• Erfolgreiche Detektion in CoIP: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• COImmunpräzipitation (IP): COIP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 229 publications below
• IHC: See 8 publications below
• IF: See 30 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

14418-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA ATP1A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP1A1 fusion protein Ag5763
• Vollständiger Name: ATPase, Na+/K+ transporting, alpha 1 polypeptide
• Berechnetes Molekulargewicht: 113 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC050359
• Gene symbol: ATP1A1
• Gene ID (NCBI): 476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.

Publikationen

Species	Application	Title
mouse	IF	Nature; Low-dose metformin targets the lysosomal AMPK pathway through PEN2.; Authors - Teng Ma
mouse	WB	Immunity; Disruption of the Na+/K+-ATPase-purinergic P2X7 receptor complex in microglia promotes stress-induced anxiety; Authors - Songqiang Huang
	WB	Adv Mater; Ultrasound Imaging of Tumor Vascular CD93 with MMRN2 Modified Microbubbles for Immune Microenvironment Prediction; Authors - Dingyi Wang
mouse	WB	ACS Nano; Biomimetic Nanomedicine Targeting Orchestrated Metabolism Coupled with Regulatory Factors to Disrupt the Metabolic Plasticity of Breast Cancer; Authors - Lingtong Meng
mouse	WB	Mol Cell; MLKL activity requires a splicing-regulated, druggable intramolecular interaction; Authors - Uris Ros
	WB	Adv Sci (Weinh); Ultrasound Responsive Nanovaccine Armed with Engineered Cancer Cell Membrane and RNA to Prevent Foreseeable Metastasis; Authors - Wenqi Sun

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jimmy (Verified Customer) (02-27-2024)

Strong signal against human cell lysate, but target protein also show up in cell membrane fraction.

• Applications: Western Blot

FH

Aleix (Verified Customer) (04-26-2022)

We tested it in immunofluorescence with skin frozen sections. It worked, although the signal is weak.

• Applications: Immunofluorescence
• Cell Tissue Type: Skin

FH

Gongsheng (Verified Customer) (12-18-2018)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human epithelium

ATP1A1 Antibody Immunofluorescence, validation (1:100 dilution) in Human epithelium (Cat no:14418-1-AP)