ATP1A1-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ATP1A1-Specific Polyklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Rezensionen (1)

Kat-Nr. : CL488-55187

Synonyme

ATP1A1, ATP1A 1, Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13

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  IF Staining of HEK-293 using CL488-55187

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using CoraLite® Plus 488 ATP1A1-Specific antibody (CL488-55187) at dilution of 1:200.

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  FC experiment of HEK-293 using CL488-55187

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ATP1A1-Specific (CL488-55187) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-55187 bindet in IF/ICC, FC (Intra) ATP1A1-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ATPase, Na+/K+ transporting, alpha 1 polypeptide
• Berechnetes Molekulargewicht: 113 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: NM_000701
• Gene symbol: ATP1A1
• Gene ID (NCBI): 476
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. This antibody is specific to ATP1A1.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Paulina (Verified Customer) (04-22-2025)

Works really well, labels cell membranes effectively without background due to secondary antibody

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: MDA-MB-231

CL Plus 488 ATP1A1-Specific Antibody Immunofluorescence validation (1:100 dilution) in MDA-MB-231 (Cat no:CL488-55187)