ATP1A2 Polyklonaler Antikörper

ATP1A2 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 16836-1-AP

Synonyme

alpha 2 ATPase, ATP1A2, FHM2, KIAA0778, MHP2, Sodium pump subunit alpha 2

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Galerie der Validierungsdaten

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  WB analysis using 16836-1-AP

  37 ℃ incubated or boiled mouse skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 16836-1-AP (ATP1A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16836-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16836-1-AP (ATP1A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 16836-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16836-1-AP (ATP1A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 16836-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16836-1-AP (ATP1A2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 16836-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16836-1-AP (ATP1A2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 16836-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16836-1-AP (ATP1A2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 16836-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16836-1-AP (ATP1A2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 16836-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16836-1-AP (ATP1A2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 16836-1-AP

  Immunofluorescent analysis of Hela cells, using ATP1A2 antibody 16836-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 16836-1-AP

  1X10^6 HeLa cells were stained with 0.5ug ATP1A2 antibody (16836-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: bei 37°C inkubiertes Mausherzgewebe, bei 37°C inkubiertes Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Hodengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.05 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IHC: See 4 publications below
• IF: See 4 publications below

Produktinformation

16836-1-AP bindet in WB, IHC, IF, FC, ELISA ATP1A2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP1A2 fusion protein Ag10515
• Vollständiger Name: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide
• Berechnetes Molekulargewicht: 1020 aa, 112 kDa
• Beobachtetes Molekulargewicht: 97-100 kDa
• GenBank-Zugangsnummer: BC052271
• Gene symbol: ATP1A2
• Gene ID (NCBI): 477
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP1A2 (Na+/K+-ATPase α-2 subunit) is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4.The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. This antibody was raised against the internal region of the human ATP1A2 and can recognizes all the isoforms of α subunit. The 65kDa band detected occasionally may be the degradation product of ATP1A2.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Homophilic ATP1A1 binding induces activin A secretion to promote EMT of tumor cells and myofibroblast activation.; Authors - Yi-Ing Chen
human	IHC	Eur J Nucl Med Mol Imaging; Tumour blood flow for prediction of human prostate cancer aggressiveness: a study with Rubidium-82 PET, MRI and Na+/K+-ATPase-density.; Authors - Mads Ryø Jochumsen
canine	WB	J Cereb Blood Flow Metab; Evaluating the involvement of cerebral microvascular endothelial Na+/K+-ATPase and Na+-K+-2Cl- co-transporter in electrolyte fluxes in an in vitro blood-brain barrier model of dehydration.; Authors - Kasper Lykke
mouse	IHC,IF	Glia; The α2β2 isoform combination dominates the astrocytic Na(+) /K(+) -ATPase activity and is rendered nonfunctional by the α2.G301R familial hemiplegic migraine type 2-associated mutation.; Authors - Anca Stoica
human	WB,IF	Biomed Pharmacother; Rebound effects of NCX3 pharmacological inhibition: A novel strategy to accelerate myelin formation in oligodendrocytes.; Authors - Mariarosaria Cammarota
human,mouse	WB,IHC,IF	J Cell Mol Med; Comprehensive analysis identified a reduction in ATP1A2 mediated by ARID3A in abdominal aortic aneurysm.; Authors - Qunhui Wang

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