ATP1B2 Polyklonaler Antikörper

ATP1B2 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 22338-1-AP

Synonyme

Adhesion molecule in glia, ATPase Na+/K+ beta 2, Sodium/potassium-dependent ATPase subunit beta-2, Sodium/potassium-transporting ATPase subunit beta-2

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  WB analysis of mouse brain using 22338-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22338-1-AP (ATP1B2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 22338-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22338-1-AP (ATP1B2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 22338-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22338-1-AP (ATP1B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of C2C12 using 22338-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 22338-1-AP (ATP1B2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 22338-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 22338-1-AP (ATP1B2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 22338-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22338-1-AP (ATP1B2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 22338-1-AP

  IP result of anti-ATP1B2 (IP:22338-1-AP, 4ug; Detection:22338-1-AP 1:1000) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of human brain using 22338-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 22338-1-AP (ATP1B2 Antibody) at dilution of 1:50.

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  IHC staining of human brain using 22338-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 22338-1-AP (ATP1B2 Antibody) at dilution of 1:50.

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  IF Staining of mouse brain using 22338-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using ATP1B2 antibody (22338-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, C2C12-Zellen, C6-Zellen, Maus-Skelettmuskelgewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IF: See 2 publications below

Produktinformation

22338-1-AP bindet in WB, IHC, IF-P, IP, ELISA ATP1B2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP1B2 fusion protein Ag17818
• Vollständiger Name: ATPase, Na+/K+ transporting, beta 2 polypeptide
• Berechnetes Molekulargewicht: 290 aa, 33 kDa
• Beobachtetes Molekulargewicht: 45-65 kDa
• GenBank-Zugangsnummer: BC126175
• Gene symbol: ATP1B2
• Gene ID (NCBI): 482
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP1B2 is the β2 subunit of Na+/K+-ATPase which is an essential membrane-bound enzyme responsible for the transport of Na+ and K+ in most eukaryotic cells. ATP1B2 is also called the adhesion molecule on glia (AMOG) and it is highly expressed in normal glia. It is a heavily glycosylated protein that plays a role in cellular adhesion in the CNS. Recently differential expression of ATP1B2 has been found in some glioneuronal tumors (PMID: 23887941, 19371356). This antibody recognizes the endogenous ATP1B2 protein in human brain. The bands between 45 kDa and 65 kDa represent the glycosylated forms of ATP1B2 in different levels (PMID: 8918259).

Publikationen

Species	Application	Title
mouse	IF	Cell Mol Gastroenterol Hepatol; Self-renewing Monolayer of Primary Colonic or Rectal Epithelial Cells.; Authors - Yuli Wang
rat	WB	Redox Biol; Caveolin 1-related autophagy initiated by aldosterone-induced oxidation promotes liver sinusoidal endothelial cells defenestration.; Authors - Xiaoying Luo
human	WB	Cancer Cell Int; Spider venom components decrease glioblastoma cell migration and invasion through RhoA-ROCK and Na+/K+-ATPase β2: potential molecular entities to treat invasive brain cancer.; Authors - Natália Barreto
human,rat	WB	World J Biol Psychiatry; Altered expression levels of miR-144-3p and ATP1B2 are associated with schizophrenia.; Authors - Bo Pan
human	WB	Eur J Appl Physiol; Resistance training upregulates skeletal muscle Na+, K+-ATPase content, with elevations in both α1 and α2, but not β isoforms.; Authors - Muath M Altarawneh
rat	WB	Neurosci Lett; Effects of icariin on alleviating schizophrenia-like symptoms by regulating the miR-144-3p/ATP1B2/mTOR signalling pathway; Authors - Bo Pan