ATP2C1 Polyklonaler Antikörper

ATP2C1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 13310-1-AP

Synonyme

ATP dependent Ca(2+) pump PMR1, ATP2C1, ATP2C1A, ATPase 2C1, HHD, hSPCA1, KIAA1347, PMR1, PMR1L, SPCA1

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Galerie der Validierungsdaten

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  WB analysis using 13310-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13310-1-AP (ATP2C1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13310-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13310-1-AP (ATP2C1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13310-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13310-1-AP (ATP2C1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 13310-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13310-1-AP (ATP2C1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 13310-1-AP

  IP Result of anti-ATP2C1 (IP:13310-1-AP, 4ug; Detection:13310-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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  IHC staining of mouse brain using 13310-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13310-1-AP (ATP2C1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13310-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13310-1-AP (ATP2C1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 13310-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13310-1-AP (ATP2C1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

13310-1-AP bindet in WB, IP, IHC, IF, ELISA ATP2C1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATP2C1 fusion protein Ag4143
• Vollständiger Name: ATPase, Ca++ transporting, type 2C, member 1
• Berechnetes Molekulargewicht: 919 aa, 101 kDa
• Beobachtetes Molekulargewicht: 95-103 kDa
• GenBank-Zugangsnummer: BC028139
• Gene symbol: ATP2C1
• Gene ID (NCBI): 27032
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP2C1, also known as hSPCA1, belongs to the family of P-type cation transport ATPases. ATP2C1 is a Golgi-localized ATPase that mediates Golgi uptake of cytosolic Ca(2+) and Mg(2+) and has a role in regulating Ca(2+) and Mn(2+) cellular content (PMID: 11741891). ATP2C1 is found in most tissues except colon, thymus, spleen, and leukocytes. It is expressed in keratinocytes (PMID: 15831496, 14632183). Defects in ATP2C1 cause Hailey-Hailey disease (HHD)(PMID: 10615129, 28035777).

Publikationen

Species	Application	Title
human	WB	Front Cell Dev Biol; A Negative Feedback Loop in Ultraviolet A-Induced Senescence in Human Dermal Fibroblasts Formed by SPCA1 and MAPK.; Authors - Hongfu Xie
human	WB	Clin Cosmet Investig Dermatol; The Pathogenic Mechanism of the ATP2C1 p.Ala109_Gln120del Mutation in Hailey-Hailey Disease; Authors - Peiyao Li
mouse	WB	BMC Pulm Med; Tetramethylpyrazine ameliorates endotoxin-induced acute lung injury by relieving Golgi stress via the Nrf2/HO-1 signaling pathway; Authors - Shaona Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (09-12-2019)

This antibody could detect endogenous ATP2C1 both for WB and IF. For WB, besides the band at the expected molecular weight, it also gives a non specific band at ~ 150 kd.

• Applications: Western Blot, Immunofluorescence,